Overview

  • Product name

  • Description

    Goat polyclonal to MPP5/Pals1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MPP5/Pals1 aa 174-187 (N terminal).
    Sequence:

    NKASPPFPLISNAQ

  • Positive control

    • Human Brain lysate
  • General notes

     This product was previously labelled as MPP5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab20511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ELISA 1/32000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).

Target

  • Function

    May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
  • Tissue specificity

    Expressed in the retina (at protein level).
  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.
  • Domain

    The L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
    The PDZ domain binds to the C-terminus of SC6A1.
  • Cellular localization

    Cell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ12615 antibody
    • MAGUK p55 subfamily member 5 antibody
    • Membrane protein palmitoylated 5 antibody
    • MPP 5 antibody
    • MPP5 antibody
    • MPP5_HUMAN antibody
    • PALS 1 antibody
    • PALS1 antibody
    • Protein associated with Lin-7 antibody
    • Stardust (Drosophila) antibody
    • Stardust antibody
    see all

Images

References

ab20511 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic dorsal forebrain)
Permeabilization
Yes - Triton 0.5%
Specification
Embryonic dorsal forebrain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 10 2016

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