Key features and details
- Rabbit polyclonal to MPP5/Pals1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MPP5/Pals1 antibody
See all MPP5/Pals1 primary antibodies
DescriptionRabbit polyclonal to MPP5/Pals1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Orangutan
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
This product was previously labelled as MPP5
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab217068 was affinity purified using an epitope specific to MPP5/Pals1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab217068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton.
Tissue specificityExpressed in the retina (at protein level).
Sequence similaritiesBelongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain.
DomainThe L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
The PDZ domain binds to the C-terminus of SC6A1.
Cellular localizationCell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon.
- Information by UniProt
- FLJ12615 antibody
- MAGUK p55 subfamily member 5 antibody
- Membrane protein palmitoylated 5 antibody
All lanes : Anti-MPP5/Pals1 antibody (ab217068) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 30 seconds
Detection of MPP5/Pals1 in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (0.5 or 1 mg for IP, 20% of IP loaded). ab217068 was used at 6 µg/mg lysate for IP (Lane 1), and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: chemiluminescence with an exposure time of 30 seconds.
ab217068 has not yet been referenced specifically in any publications.