Anti-MPP5/Pals1 antibody (ab224219)
Key features and details
- Rabbit polyclonal to MPP5/Pals1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MPP5/Pals1 antibody
See all MPP5/Pals1 primary antibodies -
Description
Rabbit polyclonal to MPP5/Pals1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Orangutan -
Immunogen
Recombinant fragment corresponding to Human MPP5/Pals1 aa 50-250.
Database link: Q8N3R9 -
Positive control
- IHC-P: Human fallopian tube tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
May play a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells. May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter. Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton. -
Tissue specificity
Expressed in the retina (at protein level). -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain. -
Domain
The L27 domain 1 functions in targeting to the tight junctions by binding to INADL.
The PDZ domain binds to the C-terminus of SC6A1. -
Cellular localization
Cell membrane. Endomembrane system. Cell junction > tight junction. Localized to the tight junctions of epithelial cells and a subset of intracellular vesicles. In the retina, detected at the outer limiting membrane (OLM), apical to the adherens junction (AJ), where it colocalizes with CRB1. Colocalizes with MPP1 in the retina at the outer limiting membrane (OLM). Localized to the Purkinje cell body and axon. - Information by UniProt
-
Database links
- Entrez Gene: 64398 Human
- Entrez Gene: 56217 Mouse
- Entrez Gene: 100171889 Orangutan
- Omim: 606958 Human
- SwissProt: Q8N3R9 Human
- SwissProt: Q9JLB2 Mouse
- SwissProt: Q5RDQ2 Orangutan
- Unigene: 652312 Human
see all -
Alternative names
- FLJ12615 antibody
- MAGUK p55 subfamily member 5 antibody
- Membrane protein palmitoylated 5 antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab224219 has not yet been referenced specifically in any publications.