Key features and details
- Rabbit polyclonal to MPP8
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MPP8 antibody
See all MPP8 primary antibodies
DescriptionRabbit polyclonal to MPP8
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
antigen sequence, corresponding to internal sequence amino acids 305-417 of Human MPP8.
- IHC: Human testis tissue and human cerebral cortex tissue ICC: U 2 OS cells WB: RT 4, U 251 MG and Human tonsil lysates
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesContains 4 ANK repeats.
Contains 1 chromo domain.
modificationsPhosphorylated in M (mitotic) phase.
- Information by UniProt
- HSMPP8 antibody
- M-phase phosphoprotein 8 antibody
- M-phase phosphoprotein, mpp antibody
ab122575, at 1/500 dilution, staining MPP8 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
All lanes : Anti-MPP8 antibody (ab122575) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
ab122575, at 1/500 dilution, staining MPP8 in paraffin-embedded Human testis tissue by Immunohistochemistry.
ab122575, at 4 µg/ml, staining MPP8 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
ab122575 has not yet been referenced specifically in any publications.