Key features and details
- Rabbit polyclonal to MPP8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MPP8 antibody
See all MPP8 primary antibodies
DescriptionRabbit polyclonal to MPP8
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide within Human MPP8 aa 400-500 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q99549
- This antibody gave a positive signal in MCF7 Nuclear and HEK293 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab124499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 97 kDa).|
Sequence similaritiesContains 4 ANK repeats.
Contains 1 chromo domain.
modificationsPhosphorylated in M (mitotic) phase.
- Information by UniProt
- HSMPP8 antibody
- M-phase phosphoprotein 8 antibody
- M-phase phosphoprotein, mpp antibody
All lanes : Anti-MPP8 antibody (ab124499) at 1 µg/ml
Lane 1 :
MCF7 nuclear extract lysate (ab14860)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa (possible non-specific binding)
Exposure time: 2 minutes
The predicted molecular weight of MPP8 is 97 kDa (SwissProt), however we expect to observe a banding pattern around 125 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab124499 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
ab124499 has not yet been referenced specifically in any publications.