Recombinant Anti-MPP8 antibody [EPR19856] (ab214812)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19856] to MPP8
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-MPP8 antibody [EPR19856]
See all MPP8 primary antibodies -
Description
Rabbit monoclonal [EPR19856] to MPP8 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type mouse embryonic stem (ES) cell lysate; RAW264.7 and L-929 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19856 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa). |
Target
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Sequence similarities
Contains 4 ANK repeats.
Contains 1 chromo domain. -
Post-translational
modificationsPhosphorylated in M (mitotic) phase. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 75339 Mouse
- SwissProt: Q3TYA6 Mouse
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Alternative names
- HSMPP8 antibody
- M-phase phosphoprotein 8 antibody
- M-phase phosphoprotein, mpp antibody
see all
Images
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All lanes : Anti-MPP8 antibody [EPR19856] (ab214812) at 1/1000 dilution
Lane 1 : Wild-type mouse embryonic stem (ES) cell lysate
Lane 2 : MPP8 knockout mouse embryonic stem (ES) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThe cell lysates were kindly provided by our collaborator Dr. Jinzhong Qin, Nanjing University MARC.
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All lanes : Anti-MPP8 antibody [EPR19856] (ab214812) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 3 minutes; Lane 2: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab214812 has not yet been referenced specifically in any publications.