Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-MPP8 antibody [EPR19856] - BSA and Azide free (ab226860)

Overview

  • Product name

    Anti-MPP8 antibody [EPR19856] - BSA and Azide free
    See all MPP8 primary antibodies
  • Description

    Rabbit monoclonal [EPR19856] to MPP8 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse MPP8 aa 1-800. The exact sequence is proprietary.
    Database link: Q3TYA6

  • Positive control

    • WB: Wild-type mouse embryonic stem (ES) cell lysate.
  • General notes

    Ab226860 is the carrier-free version of ab214812. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226860 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19856
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa).

Target

  • Sequence similarities

    Contains 4 ANK repeats.
    Contains 1 chromo domain.
  • Post-translational
    modifications

    Phosphorylated in M (mitotic) phase.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • HSMPP8 antibody
    • M-phase phosphoprotein 8 antibody
    • M-phase phosphoprotein, mpp antibody
    • M-phase phosphoprotein, mpp8 antibody
    • MPHOSPH8 antibody
    • MPP8_HUMAN antibody
    • RP11-523H24.1 antibody
    • Twa3 antibody
    • Two hybrid-associated protein 3 with RanBPM antibody
    see all

Images

  • All lanes : Anti-MPP8 antibody [EPR19856] (ab214812) at 1/1000 dilution

    Lane 1 : Wild-type mouse embryonic stem (ES) cell lysate
    Lane 2 : MPP8 knockout mouse embryonic stem (ES) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    The cell lysates were kindly provided by our collaborator Dr. Jinzhong Qin, Nanjing University MARC.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214812).

References

ab226860 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab226860.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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