Product nameAnti-MPP8 antibody [EPR19856] - BSA and Azide free
See all MPP8 primary antibodies
DescriptionRabbit monoclonal [EPR19856] to MPP8 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse MPP8 aa 1-800. The exact sequence is proprietary.
Database link: Q3TYA6
- WB: Wild-type mouse embryonic stem (ES) cell lysate.
Ab226860 is the carrier-free version of ab214812. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab226860 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab226860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 110 kDa (predicted molecular weight: 97 kDa).|
Sequence similaritiesContains 4 ANK repeats.
Contains 1 chromo domain.
modificationsPhosphorylated in M (mitotic) phase.
- Information by UniProt
- HSMPP8 antibody
- M-phase phosphoprotein 8 antibody
- M-phase phosphoprotein, mpp antibody
All lanes : Anti-MPP8 antibody [EPR19856] (ab214812) at 1/1000 dilution
Lane 1 : Wild-type mouse embryonic stem (ES) cell lysate
Lane 2 : MPP8 knockout mouse embryonic stem (ES) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
The cell lysates were kindly provided by our collaborator Dr. Jinzhong Qin, Nanjing University MARC.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214812).
ab226860 has not yet been referenced specifically in any publications.