The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: 1-4 µg/mg lysate.
WB: 1/2500 - 1/25000. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
Present in rapidly proliferating cell lines.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Detection of Human Mps1 by Western Blot and Immunoprecipitation.
Samples: Whole cell lysate (15 and 50 µg for A; 50 µg for B; 2 mg for B) from HeLa cells. In B, HeLa cells were treated with an siRNA for vimentin (control) or Mps1 Ab24226 used at 0.033 (A) and 0.33 (B) µg/ml for WB and at 1 µg/2 mg lysate for IP (C). Mps1 was also immunoprecipitated with Ab24226 using 1 µg/2 mg lysate. IP reactions were controlled using normal rabbit IgG (control IP). Immunoprecipitated Mps1 was blotted using a goat antibody directed to an upstream epitope on Mps1. Detection: Chemiluminescence with exposure times of 1 minute (A), 15 seconds (B) and 30 seconds (C).