ab24227 reacts with TTK (MPS1) protein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
Present in rapidly proliferating cell lines.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Lane 1 : HeLa cell lyste at 15µg and ab at 0.033ug/ml Lane 2 : HeLa cell lyste at 50ug and ab at 0.033ug/ml Lanes 3-4 : HeLa cell lyste at 50ug and ab at 0.33ug/ml Lanes 5-7 : HeLa cell lyste at 2mg and ab at 1ug/2mg lysate
Developed using the ECL technique.
Predicted band size: 95 kDa Observed band size: 95 kDa
ECL exposure time: A: 1min B: 15 second C: 30 second
ICC/IF image of ab24227 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24227, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.