Anti-TTK/Mps1 antibody [OTI13E9] (ab236525)
Key features and details
- Mouse monoclonal [OTI13E9] to TTK/Mps1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-TTK/Mps1 antibody [OTI13E9]
See all TTK/Mps1 primary antibodies -
Description
Mouse monoclonal [OTI13E9] to TTK/Mps1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Recombinant fragment corresponding to Human TTK/Mps1 aa 150-500. (NP_003309) produced in E.coli.
Database link: P33981 -
Positive control
- WB: K562 and Raji cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI13E9 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Predicted molecular weight: 97 kDa.
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Notes |
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WB
1/500. Predicted molecular weight: 97 kDa. |
Target
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Function
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint. -
Tissue specificity
Present in rapidly proliferating cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 7272 Human
- Omim: 604092 Human
- SwissProt: Q4R945 Cynomolgus monkey
- SwissProt: P33981 Human
- Unigene: 169840 Human
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Alternative names
- cancer/testis antigen 96 antibody
- CT96 antibody
- Dual specificity protein kinase TTK antibody
see all
Images
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All lanes : Anti-TTK/Mps1 antibody [OTI13E9] (ab236525) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 97 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236525 has not yet been referenced specifically in any publications.