Anti-MR1 antibody (ab229987)
Key features and details
- Rabbit polyclonal to MR1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MR1 antibody
See all MR1 primary antibodies -
Description
Rabbit polyclonal to MR1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human MR1 aa 150-253.
Database link: Q95460 -
Positive control
- WB: THP-1 and K562 whole cell lysates; Mouse brain lysate. IHC-P: Human liver and kidney tissues. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 39 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. Involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain called mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and therefore may be involved in monitoring commensal flora or serve as a distress signal. Expression and MAIT cell recognition seem to be ligand-dependent. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Domain
The alpha-3 region and to a lesser extent the transmembrane and cytosolic domains regulate surface expression. The alpha-3 region mediates interaction with B2M (PubMed:23051753).
The ligand-binding groove is ideally suited to present small organic compounds that can originate from vitamins rather than antigenic peptides. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted; Cell membrane. Endoplasmic reticulum and Cell membrane. Endoplasmic reticulum membrane. The larger proportion remains in the ER in an immature state. The subset that reach cell surface does it through a B2M-independent pathway. - Information by UniProt
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Database links
- Entrez Gene: 3140 Human
- Entrez Gene: 15064 Mouse
- Omim: 600764 Human
- SwissProt: Q95460 Human
- SwissProt: O19478 Mouse
- SwissProt: Q8HWB0 Mouse
- Unigene: 13500 Human
- Unigene: 7580 Mouse
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Alternative names
- Class I histocompatibility antigen like protein antibody
- Class I histocompatibility antigen-like protein antibody
- HLALS antibody
see all
Images
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Paraffin-embedded human kidney tissue stained for MR1 using ab229987 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-MR1 antibody (ab229987) at 1/1000 dilution
Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 3 : Mouse brain lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa -
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for MR1 using ab229987at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human liver tissue stained for MR1 using ab229987 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229987 has been referenced in 1 publication.
- Cao X et al. Effects of Probiotics BaSC06 on Intestinal Digestion and Absorption, Antioxidant Capacity, Microbiota Composition, and Macrophage Polarization in Pigs for Fattening. Front Vet Sci 7:570593 (2020). PubMed: 33240950