Overview

  • Product name

  • Description

    Rabbit polyclonal to MRAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 57-86 of Human MRAP2 (NP_612418.2)

  • Positive control

    • 293 cell lysate; Human brain tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab129397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 24 kDa.
IHC-P 1/10 - 1/50.

Target

Images

  • Anti-MRAP2 antibody (ab129397) at 1/100 dilution + 293 cell lysate at 35 µg

    Predicted band size: 24 kDa

  • ab129397 at 1/10 dilution labelling MRAP2 in Human brain tissue by immunohistochemistry (paraffin-embedded, formalin fixed). Primary incubation was followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab129397 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - other (hypothalamus spine)
Loading amount
50 µg
Specification
hypothalamus spine
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Dec 18 2012

Answer

DISCOUNT CODE: *******
Expiration date: DD MM YYYY
Value: £227.00
I am very pleased to hear you would like to accept our offer and test ab129397 in Mouse. This code will give you £227.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you for contacting us.
Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.
As shown on the product datasheet the anti-MRAP2 antibody ab129397 has been tested in Western Blot and in IHC-P on Human brain tissue slides. However, to our knowledge, it has not been tested in Mouse. It is likely that ab129397 is suitable for Mouse since the homology between the immunogen and the murine MRAP2 is 97%.
By participating in our AbTrial program you can now use our products in an untested application or species without financial risk.
Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.

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