Key features and details
- Rabbit polyclonal to MRas
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab26303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa). Block with 5% milk and dilute in TBST only.|
FunctionMay serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
Tissue specificityExpression highly restricted to the brain and heart.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
Cellular localizationCell membrane.
- Information by UniProt
- FLJ42964 antibody
- M ras antibody
- M-ras antibody
ICC/IF image of ab26303 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26303, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-MRas antibody (ab26303)
Lane 1 : HEK 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate
All lanes : anti-rabbit HRP conjugated
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Observed band larger than predicted size, thought to be due to post-translational modifications.
ab26303 has not yet been referenced specifically in any publications.