Product nameAnti-MRas antibody
See all MRas primary antibodies
DescriptionRabbit polyclonal to MRas
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- HeLa or HEK293 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab26303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 24 kDa). Block with 5% milk and dilute in TBST only.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionMay serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
Tissue specificityExpression highly restricted to the brain and heart.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
Cellular localizationCell membrane.
- Information by UniProt
- FLJ42964 antibody
- M ras antibody
- M-ras antibody
All lanes : Anti-MRas antibody (ab26303)
Lane 1 : HEK 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate
All lanes : anti-rabbit HRP conjugated
Developed using the ECL technique.
Predicted band size: 24 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Observed band larger than predicted size, thought to be due to post-translational modifications.
ICC/IF image of ab26303 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26303, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab26303 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab26303, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab26303 has not yet been referenced specifically in any publications.