Overview

  • Product name
    Anti-MRas antibody [EPR12457]
    See all MRas primary antibodies
  • Description
    Rabbit monoclonal [EPR12457] to MRas
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MRas aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O14807

  • Positive control
    • Fetal brain and fetal heart lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab176570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      May serve as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Weakly activates the MAP kinase pathway.
    • Tissue specificity
      Expression highly restricted to the brain and heart.
    • Sequence similarities
      Belongs to the small GTPase superfamily. Ras family.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ42964 antibody
      • M ras antibody
      • M-ras antibody
      • Mras antibody
      • Muscle and microspikes Ras antibody
      • Muscle RAS oncogene homolog antibody
      • Muscle Ras oncogene homologue antibody
      • Muscle Ras viral oncogene homolog antibody
      • R ras3 antibody
      • R-ras3 antibody
      • Ras related protein MRas antibody
      • Ras related protein RRas3 antibody
      • Ras-related protein M-Ras antibody
      • Ras-related protein R-Ras3 antibody
      • RASM_HUMAN antibody
      • Related Ras viral oncogene homolog 3 antibody
      • RRas3 antibody
      • XRas antibody
      see all

    Images

    • All lanes : Anti-MRas antibody [EPR12457] (ab176570) at 1/1000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 24 kDa

    • Western blot analysis on immunoprecipitation pellet from (1)  Fetal brain lysate or (2) 1XPBS (negative control) using ab176570 at a 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab176570 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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