Product nameAnti-MRC2/ENDO180 antibody
See all MRC2/ENDO180 primary antibodies
DescriptionRabbit polyclonal to MRC2/ENDO180
ab70132 detects endogenous levels of total MRC2/ENDO180 protein.
Tested applicationsSuitable for: Flow Cyt, WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MRC2/ENDO180 (internal sequence).
- HepG2 cells, 293 cell extracts and human brain tissue.
This product was previously labelled as MRC2
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab70132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/5 (Abreview).
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 167 kDa (predicted molecular weight: 167 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs).
Tissue specificityUbiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney.
Sequence similaritiesContains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain.
DomainC-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner.
Fibronectin type-II domain mediates collagen-binding.
Ricin B-type lectin domain contacts with the second C-type lectin domain.
- Information by UniProt
- C-type lectin domain family 13 member E antibody
- C-type mannose receptor 2 antibody
- CD 280 antibody
All lanes : Anti-MRC2/ENDO180 antibody (ab70132) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : 293 cell extracts with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 167 kDa
Observed band size: 167 kDa
ab70132, at a 1/500 dilution, staining MRC2/ENDO180 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
ab70132, at a 1/50 dilution, staining MRC2/ENDO180 in paraffin embedded human brain tissue by Immunohistochemistry, in the absence or presence of the immunising peptide.
ab70132 staining MRC2/ENDO180 in tonsillar tissue by Flow Cytometry. Gating strategy : Viable leukocytes gated in FSC/SSC. The sample was incubated with the primary antibody (dilution 1/5 in PBS/0.5%BSA/2.5mM EDTA), for 30 minutes at 4°C. An FITC-conjugated goat polyclonal to Rabbit IgG (dilution 1/50) was used as secondary antibody.
ab70132 staining MRC2/ENDO180 in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Inhibitor CM for 4 minutes at 37°C; antigen retrieval was by heat mediation in Tris/Borate/EDTA buffer pH 8. Samples were incubated with primary antibody (1/50 ) for 32 minutes at 37°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This product has been referenced in:
- Wohlfahrt T et al. PU.1 controls fibroblast polarization and tissue fibrosis. Nature 566:344-349 (2019). Read more (PubMed: 30700907) »
- Hsiung S et al. Hyperglycemia does not affect tissue repair responses in shear stress-induced atherosclerotic plaques in ApoE-/- mice. Sci Rep 8:7530 (2018). Read more (PubMed: 29760458) »