Overview

  • Product name

    Anti-MRC2/ENDO180 antibody
    See all MRC2/ENDO180 primary antibodies
  • Description

    Rabbit polyclonal to MRC2/ENDO180
  • Host species

    Rabbit
  • Specificity

    ab70132 detects endogenous levels of total MRC2/ENDO180 protein.

  • Tested applications

    Suitable for: Flow Cyt, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MRC2/ENDO180 (internal sequence).

  • Positive control

    • HepG2 cells, 293 cell extracts and human brain tissue.
  • General notes

     This product was previously labelled as MRC2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
WB
ELISA
IHC-P
ICC/IF
  • Application notes
    ELISA: 1/20000.
    Flow Cyt: 1/5 (Abreview).
    ICC/IF: 1/500 - 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 167 kDa (predicted molecular weight: 167 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs).
    • Tissue specificity

      Ubiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney.
    • Sequence similarities

      Contains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • Domain

      C-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner.
      Fibronectin type-II domain mediates collagen-binding.
      Ricin B-type lectin domain contacts with the second C-type lectin domain.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C-type lectin domain family 13 member E antibody
      • C-type mannose receptor 2 antibody
      • CD 280 antibody
      • CD280 antibody
      • CD280 antigen antibody
      • CLEC 13E antibody
      • CLEC13E antibody
      • ENDO 180 antibody
      • ENDO180 antibody
      • Endocytic receptor (macrophage mannose receptor family) antibody
      • Endocytic receptor 180 antibody
      • FLJ35911 antibody
      • KIAA0709 antibody
      • Macrophage mannose receptor 2 antibody
      • Mannose receptor C type 2 antibody
      • MRC 2 antibody
      • MRC2 antibody
      • MRC2_HUMAN antibody
      • UPAR-associated protein antibody
      • UPARAP antibody
      • Urokinase plasminogen activator receptor associated protein antibody
      • Urokinase receptor associated protein antibody
      • Urokinase receptor-associated protein antibody
      • Urokinase-type plasminogen activator receptor-associated protein antibody
      see all

    Images

    • All lanes : Anti-MRC2/ENDO180 antibody (ab70132) at 1/500 dilution

      Lane 1 : 293 cell extracts
      Lane 2 : 293 cell extracts with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 167 kDa
      Observed band size: 167 kDa

    • ab70132, at a 1/500 dilution, staining MRC2/ENDO180 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

    • ab70132, at a 1/50 dilution, staining MRC2/ENDO180 in paraffin embedded human brain tissue by Immunohistochemistry, in the absence or presence of the immunising peptide.

    • ab70132 staining MRC2/ENDO180 in tonsillar tissue by Flow Cytometry. Gating strategy : Viable leukocytes gated in FSC/SSC. The sample was incubated with the primary antibody (dilution 1/5 in PBS/0.5%BSA/2.5mM EDTA), for 30 minutes at 4°C. An FITC-conjugated goat polyclonal to Rabbit IgG (dilution 1/50) was used as secondary antibody.

      See Abreview

    • ab70132 staining MRC2/ENDO180 in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Inhibitor CM for 4 minutes at 37°C; antigen retrieval was by heat mediation in Tris/Borate/EDTA buffer pH 8. Samples were incubated with primary antibody (1/50 ) for 32 minutes at 37°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:

    • Wohlfahrt T  et al. PU.1 controls fibroblast polarization and tissue fibrosis. Nature 566:344-349 (2019). Read more (PubMed: 30700907) »
    • Hsiung S  et al. Hyperglycemia does not affect tissue repair responses in shear stress-induced atherosclerotic plaques in ApoE-/- mice. Sci Rep 8:7530 (2018). Read more (PubMed: 29760458) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreatic Cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/Borate/EDTA buffer pH 8
    Permeabilization
    No
    Specification
    Pancreatic Cancer
    Blocking step
    Inhibitor CM as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 19 2012

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (pancreatic cancer cell line)
    Permeabilization
    Yes - 0.3 % triton X-100 in PBS
    Specification
    pancreatic cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 11 2012

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Pancreatic Cancer)
    Loading amount
    20 µg
    Specification
    Pancreatic Cancer
    Treatment
    5ng/ml TGF-beta for 72 hours
    Gel Running Conditions
    Reduced Denaturing (4-12% polyacrylamide gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 05 2012

    Answer

    Thank you for contacting Abcam about ab70132 (anti-human MRC2), Lot GR2002-1. We guarantee all our antibodies to be stable for: -6 months at 4C -5 years at -20C -10 years at -80C. Unfortunately we do not have anymore of that particular lot available. However we do our best to make sure that there is very minimal batch variability. If there is anything else I can help you with, please feel free to c

    Read More
    Application
    Flow Cytometry
    Sample
    Human Cell (tonsillar tissue)
    Specification
    tonsillar tissue
    Fixation
    None
    Permeabilization
    No
    Gating Strategy
    Viable leukocytes gated in FSC/SSC

    Abcam user community

    Verified customer

    Submitted Nov 10 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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