Anti-MRC2/ENDO180 antibody (ab70132)
- Datasheet
- References (9)
- Protocols
Overview
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Product name
Anti-MRC2/ENDO180 antibody
See all MRC2/ENDO180 primary antibodies -
Description
Rabbit polyclonal to MRC2/ENDO180 -
Host species
Rabbit -
Specificity
ab70132 detects endogenous levels of total MRC2/ENDO180 protein.
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Tested applications
Suitable for: Flow Cyt, WB, ELISA, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human MRC2/ENDO180 (internal sequence).
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Positive control
- HepG2 cells, 293 cell extracts and human brain tissue.
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General notes
This product was previously labelled as MRC2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab70132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | ||
| WB | ||
| ELISA | ||
| IHC-P | ||
| ICC/IF |
Flow Cyt: 1/5 (Abreview).
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 167 kDa (predicted molecular weight: 167 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
May play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May play a role during cancer progression as well as in other chronic tissue destructive diseases acting on collagen turnover. May participate in remodeling of extracellular matrix co-operating with the matrix metalloproteinases (MMPs). -
Tissue specificity
Ubiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney. -
Sequence similarities
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
Domain
C-type lectin domains 3 to 8 are not required for calcium-dependent binding of mannose, fucose and N-acetylglucosamine. C-type lectin domain 2 is responsible for sugar-binding in a calcium-dependent manner.
Fibronectin type-II domain mediates collagen-binding.
Ricin B-type lectin domain contacts with the second C-type lectin domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9902 Human
- Entrez Gene: 17534 Mouse
- Entrez Gene: 498011 Rat
- Omim: 612264 Human
- SwissProt: Q9UBG0 Human
- SwissProt: Q64449 Mouse
- SwissProt: Q4TU93 Rat
- Unigene: 7835 Human
see all -
Alternative names
- C-type lectin domain family 13 member E antibody
- C-type mannose receptor 2 antibody
- CD 280 antibody
see all
Images
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Western blot - Anti-MRC2/ENDO180 antibody (ab70132)All lanes : Anti-MRC2/ENDO180 antibody (ab70132) at 1/500 dilution
Lane 1 : 293 cell extracts
Lane 2 : 293 cell extracts with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 167 kDa
Observed band size: 167 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-MRC2/ENDO180 antibody (ab70132)ab70132, at a 1/500 dilution, staining MRC2/ENDO180 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody (ab70132)ab70132, at a 1/50 dilution, staining MRC2/ENDO180 in paraffin embedded human brain tissue by Immunohistochemistry, in the absence or presence of the immunising peptide.
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Flow Cytometry - Anti-MRC2/ENDO180 antibody (ab70132)This image is courtesy of an anonymous Abreviewab70132 staining MRC2/ENDO180 in tonsillar tissue by Flow Cytometry. Gating strategy : Viable leukocytes gated in FSC/SSC. The sample was incubated with the primary antibody (dilution 1/5 in PBS/0.5%BSA/2.5mM EDTA), for 30 minutes at 4°C. An FITC-conjugated goat polyclonal to Rabbit IgG (dilution 1/50) was used as secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRC2/ENDO180 antibody (ab70132)This image is courtesy of an anonymous Abreviewab70132 staining MRC2/ENDO180 in Human pancreatic cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Inhibitor CM for 4 minutes at 37°C; antigen retrieval was by heat mediation in Tris/Borate/EDTA buffer pH 8. Samples were incubated with primary antibody (1/50 ) for 32 minutes at 37°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wohlfahrt T et al. PU.1 controls fibroblast polarization and tissue fibrosis. Nature 566:344-349 (2019). Read more (PubMed: 30700907) »
- Hsiung S et al. Hyperglycemia does not affect tissue repair responses in shear stress-induced atherosclerotic plaques in ApoE-/- mice. Sci Rep 8:7530 (2018). Read more (PubMed: 29760458) »
