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    mre11-antibody-12d7-ab214.pdf

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Epigenetics and Nuclear Signaling Chromosome Structure Telomeres
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Anti-Mre11 antibody [12D7] (ab214)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (71)

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Western blot - Anti-Mre11 antibody [12D7] (ab214)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
  • Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] (ab214)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
  • Western blot - Anti-Mre11 antibody [12D7] (ab214)

Key features and details

  • Mouse monoclonal [12D7] to Mre11
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human Mre11 protein (ab114277)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Mre11 antibody [12D7]
    See all Mre11 primary antibodies
  • Description

    Mouse monoclonal [12D7] to Mre11
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Amino acids 182-582 of Mre11 expressed in E. coli.

  • Positive control

    • WB HEK-293T, A431, HeLa, HepG2, PC-12 whole cell lysate; ICC: HeLa cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    12D7
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Telomeres
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Non Homol. End Joining
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human Mre11 protein (ab114277)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
WB
Rat
Human
Application Abreviews Notes
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF (2)
1/100 - 1/1000.
WB (4)
1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

(see Robinson et al).

Notes
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).

(see Robinson et al).

Target

  • Function

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • Involvement in disease

    Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • Sequence similarities

    Belongs to the MRE11/RAD32 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Target information above from: UniProt accession P49959 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4361 Human
    • Entrez Gene: 64046 Rat
    • Omim: 600814 Human
    • SwissProt: P49959 Human
    • SwissProt: Q9JIM0 Rat
    • Unigene: 192649 Human
    • Unigene: 209040 Rat
    • Alternative names

      • AT like disease antibody
      • Ataxia telangiectasia disorder like antibody
      • ATLD antibody
      • DNA recombination and repair protein antibody
      • Double strand break repair protein MRE11A antibody
      • Double-strand break repair protein MRE11A antibody
      • endo/exonuclease Mre11 antibody
      • HNGS1 antibody
      • meiotic recombination (S. cerevisiae) 11 homolog A antibody
      • Meiotic recombination 11 homolog 1 antibody
      • meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • Meiotic recombination 11 homolog A antibody
      • MmMRE11A antibody
      • Mre 11 antibody
      • MRE 11a antibody
      • MRE 11b antibody
      • MRE11 homolog 1 antibody
      • MRE11 homolog A antibody
      • MRE11 homolog double strand break repair nuclease antibody
      • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
      • MRE11 meiotic recombination 11 homolog A antibody
      • MRE11_HUMAN antibody
      • MRE11A antibody
      • MRE11b antibody
      • OTTHUMP00000236830 antibody
      • OTTHUMP00000236831 antibody
      • OTTHUMP00000236832 antibody
      • OTTHUMP00000236833 antibody
      see all

    Images

    • Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Western blot - Anti-Mre11 antibody [12D7] (ab214)
      All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : anti-mouse IgG HRP-conjugated antibody

      Predicted band size: 79 kDa



      7.5% SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
      Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)

      Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.

    • Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)
      Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)

      Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

      Secondary
      Anti-mouse IgG HRP-conjugated antibody

      Developed using the ECL technique.

      Predicted band size: 79 kDa



      7.5% SDS-PAGE

      The signal was developed with Trident ECL plus-Enhanced.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Western blot - Anti-Mre11 antibody [12D7] (ab214)
      All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution

      Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : Human Mre-11-transfected HEK-293T whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : anti-mouse IgG HRP-conjugated antibody

      Predicted band size: 79 kDa



      This image was generated using the ascites version of the product.

      7.5% SDS-PAGE

    • Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)
      Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.

      Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
      (Panel D - negative control).

      This image was generated using the ascites version of the product.

    • Western blot - Anti-Mre11 antibody [12D7] (ab214)
      Western blot - Anti-Mre11 antibody [12D7] (ab214)This image is courtesy of an anonymous Abreview
      All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution

      Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
      Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 79 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      This image was generated using the ascites version of the product.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (71)

    Publishing research using ab214? Please let us know so that we can cite the reference in this datasheet.

    ab214 has been referenced in 71 publications.

    • Mooser C  et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
    • Onn L  et al. SIRT6 is a DNA double-strand break sensor. Elife 9:N/A (2020). PubMed: 31995034
    • Thuault S  et al. A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci Rep 10:6787 (2020). PubMed: 32321993
    • Wang J  et al. Elevated MRE11 expression associated with progression and poor outcome in prostate cancer. J Cancer 10:4333-4340 (2019). PubMed: 31413753
    • Nieminuszczy J  et al. EXD2 Protects Stressed Replication Forks and Is Required for Cell Viability in the Absence of BRCA1/2. Mol Cell 75:605-619.e6 (2019). PubMed: 31255466
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 antibody [12D7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (LLC-PK1 cell line)
    Permeabilization
    Yes - 0.5% Triton-X 100
    Specification
    LLC-PK1 cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: R.T°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-Mre11 [12D7] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Human Tissue sections (Normal or gastric cancer tissues)
    Specification
    Normal or gastric cancer tissues
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 20 2014

    Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample
    Mouse Cultured Cells (Mouse embryonic fibroblasts)
    Specification
    Mouse embryonic fibroblasts
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 19 2014

    Immunocytochemistry abreview for Anti-Mre11 [12D7] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Human Cultured Cells (Hela cell line)
    Specification
    Hela cell line
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 18 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 [12D7] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample
    Human Cell (Human colon cancer cell lines)
    Specification
    Human colon cancer cell lines
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 15 2013

    Western blot abreview for Anti-Mre11 antibody [12D7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Sample
    Mouse Cell lysate - whole cell (Mouse breast cancer cell line)
    Specification
    Mouse breast cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 15 2013

    Western blot abreview for Anti-Mre11 antibody [12D7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Loading amount
    15 µg
    Specification
    Mouse embryonic fibroblasts
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 08 2011

    Western blot abreview for Anti-Mre11 antibody [12D7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (human cancer cells (colon, bladder))
    Loading amount
    20 µg
    Specification
    human cancer cells (colon, bladder)
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 12 2011

    Western blot abreview for Anti-Mre11 antibody [12D7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    20 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (10% Tris-Gly gel)
    Blocking step
    LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 17 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mre11 antibody [12D7]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal Bronchus)
    Specification
    Normal Bronchus
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 15 2007

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