Anti-Mre11 antibody [12D7] (ab214)
![Western blot - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.com/ps/products/0/ab214/Images/ab214-397811-anti-mre11-antibody-wb-a431-hela-hek293t-hepg2-cells.jpg "Western blot - Anti-Mre11 antibody [12D7] (ab214)")
Key features and details
- Mouse monoclonal [12D7] to Mre11
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Mre11 antibody [12D7]
See all Mre11 primary antibodies -
Description
Mouse monoclonal [12D7] to Mre11 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB RatHuman
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Immunogen
Amino acids 182-582 of Mre11 expressed in E. coli.
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Positive control
- WB HEK-293T, A431, HeLa, HepG2, PC-12 whole cell lysate; ICC: HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 10th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
12D7 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
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| ICC/IF |
Human
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| WB |
Rat
Human
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| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (2) |
1/100 - 1/1000.
|
| WB | (4) |
1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
(see Robinson et al). |
| Notes |
|---|
|
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). (see Robinson et al). |
Target
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Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
Involvement in disease
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. -
Sequence similarities
Belongs to the MRE11/RAD32 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
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Database links
- Entrez Gene: 4361 Human
- Entrez Gene: 64046 Rat
- Omim: 600814 Human
- SwissProt: P49959 Human
- SwissProt: Q9JIM0 Rat
- Unigene: 192649 Human
- Unigene: 209040 Rat
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Alternative names
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
see all
Images
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Western blot - Anti-Mre11 antibody [12D7] (ab214)All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : anti-mouse IgG HRP-conjugated antibody
Predicted band size: 79 kDa7.5% SDS-PAGE
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![Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.com/ps/products/0/ab214/Images/ab214-397810-anti-mre11-antibody-icc-hela-cells.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)Immunocytochemical analysis of, 4% paraformaldehyde-fixed at RT for 15 min, HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mre-11 (green) with ab214 at 1/200 dilution. Blue: Hoechst 33342 staining. Scale bar= 10 μm.
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![Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.com/ps/products/0/ab214/Images/ab214-3429-anti-mre11-antibody-12d7-flow-cytometry.jpg)
Flow Cytometry - Anti-Mre11 antibody [12D7] (ab214)Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.com/ps/products/0/ab214/Images/ab214-278591-anti-mre11-antibody-wb-pc12-cells.JPG)
Western blot - Anti-Mre11 antibody [12D7] (ab214)Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Secondary
Anti-mouse IgG HRP-conjugated antibody
Developed using the ECL technique.
Predicted band size: 79 kDa7.5% SDS-PAGE
The signal was developed with Trident ECL plus-Enhanced.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.com/ps/products/0/ab214/Images/ab214-275735-anti-mre11-antibody-12d7-western-blot.png)
Western blot - Anti-Mre11 antibody [12D7] (ab214)All lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Human Mre-11-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : anti-mouse IgG HRP-conjugated antibody
Predicted band size: 79 kDaThis image was generated using the ascites version of the product.
7.5% SDS-PAGE
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![Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.com/ps/products/0/ab214/Images/abcam214_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Mre11 antibody [12D7] (ab214)Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
(Panel D - negative control).This image was generated using the ascites version of the product.
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![Western blot - Anti-Mre11 antibody [12D7] (ab214)](https://www.abcam.com/ps/products/0/ab214/Images/ab214-208986-anti-mre11-antibody-12d7-western-blot.jpg)
Western blot - Anti-Mre11 antibody [12D7] (ab214)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Mre11 antibody [12D7] (ab214) at 1/500 dilution
Lane 1 : Mouse breast cancer cell line - whole cell lysate. Transfected with vector control.
Lane 2 : Mouse breast cancer cell line - whole cell lysate. Transfected with knockdown shRNA targeting Mre11 gene.
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-mouse IgG polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (71)
ab214 has been referenced in 71 publications.
- Mooser C et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
- Onn L et al. SIRT6 is a DNA double-strand break sensor. Elife 9:N/A (2020). PubMed: 31995034
- Thuault S et al. A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci Rep 10:6787 (2020). PubMed: 32321993
- Wang J et al. Elevated MRE11 expression associated with progression and poor outcome in prostate cancer. J Cancer 10:4333-4340 (2019). PubMed: 31413753
- Nieminuszczy J et al. EXD2 Protects Stressed Replication Forks and Is Required for Cell Viability in the Absence of BRCA1/2. Mol Cell 75:605-619.e6 (2019). PubMed: 31255466