Anti-Mre11 antibody [12D7] (ab214)

Western blot of PC12 (brain)whole cell extracts at 30µg per lane, labeling Mre11 with ab214 at 1:500. A HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

ab214 staining Mre11 in human normal and gastric cancer tissue sections (20µm) by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde for 3 hours, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/200 in 3% BSA + 0.1% Triton X-100 in TBS buffer) for 12 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

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Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells.
(Panel D - negative control).

This picture was kindly supplied as part of the reviews for ab214 and for ab89 submitted by Anya Polischouk.

The bands represent nuclear (N) and cytoplasmic (C) extract from human lung cancer cells (U1810).

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Overlay histogram showing HeLa cells stained with ab214 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab214, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.