Anti-Mre11 antibody (ab3621)

Reviews (1)Q&A (1)References (1)

Overview

  • Product name

    Anti-Mre11 antibody
    See all Mre11 primary antibodies

  • Description

    Rabbit polyclonal to Mre11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Ferret, Gorilla, Opossum, Bat

  • Immunogen

    Synthetic peptide (Human) - which represents a portion of Meiotic Recombination 11 (Mre 11)encoded within exon 3.

  • Positive control

    • HeLa cell nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

  • Involvement in disease

    Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.

  • Sequence similarities

    Belongs to the MRE11/RAD32 family.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 homolog double strand break repair nuclease antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody (ab3621)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody (ab3621)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Mre11 with ab3621 at 1/1000 (1µg/ml). Detection: DAB.
  • Western blot - Anti-Mre11 antibody (ab3621)
    Western blot - Anti-Mre11 antibody (ab3621)

    Sample: Nuclear extract (25µg) from Hela cells

    Affinity purified Rabbit anti-Mre11 (ab3621) used at 1:4,000 dilution

     

     

    Sample: Nuclear extract (25µg) from Hela cells

    Affinity purified Rabbit anti-Mre11 (ab3621) used at 1:4,000 dilution

References

This product has been referenced in:

  • Hematulin A  et al. NBS1 is required for IGF-1 induced cellular proliferation through the Ras/Raf/MEK/ERK cascade. Cell Signal 20:2276-2285 (2008). Read more (PubMed: 18793719) »
See 1 Publication for this product

Publishing research using ab3621? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Hi,
I'm interested in your anti-Mre11 antibody (ab3621). I would want to know the targeted sequence in the human protein, please. If it were propietary, could you help me by performing a BLASTp of the recognized epitope with the bovine MRE11A (NM_001192605.1) and then send me the results of the matching as % max ident, % positives, and number of gaps, please. We want to assure that your antibody will accurately cross-react with the bovine protein.
Thks a lot

Read More
Answer

Thank you for your enquiry regarding ab3621.

I can confirm that ab3621 was produced using a peptide identical for human and bovine. Since its cross-reactivity has not been tested with bovine (only on human samples) this is an untested species and we do not guarantee it yet.

I could offer you a discount code for testing if you wish to test one. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.


If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testab3621 in bovine. I will then send a discount code. This code must be issued before purchasingab3621 so please wait for my reply before ordering.

2. Purchaseab3621 either by phone, fax, or online (www.abcam.com).

3. Test it in bovine.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifab3621 turns out to be unsuitable for bovine, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mre11 antibody

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa and Indian muntjac)
Specification
HeLa and Indian muntjac
Fixative
Paraformaldehyde
Read More

Dr. Kirk Mcmanus

Verified customer

Submitted Apr 13 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com