Anti-Mre11 antibody (ab3621)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Mre11 antibody
See all Mre11 primary antibodies -
DescriptionRabbit polyclonal to Mre11
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Ferret, Gorilla, Opossum, Bat
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Immunogen
Synthetic peptide (Human) - which represents a portion of Meiotic Recombination 11 (Mre 11)encoded within exon 3.
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Positive control
- HeLa cell nuclear extract.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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FunctionComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
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Involvement in diseaseDefects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
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Sequence similaritiesBelongs to the MRE11/RAD32 family.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
- Information by UniProt
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Database links
- Entrez Gene: 4361 Human
- Entrez Gene: 17535 Mouse
- Entrez Gene: 64046 Rat
- Omim: 600814 Human
- SwissProt: P49959 Human
- SwissProt: Q61216 Mouse
- SwissProt: Q9JIM0 Rat
- Unigene: 192649 Human
see all -
Alternative names
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody (ab3621)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Mre11 with ab3621 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - Anti-Mre11 antibody (ab3621)Sample: Nuclear extract (25
µ g) from Hela cellsAffinity purified Rabbit anti-Mre11 (ab3621) used at 1:4,000 dilution
Sample: Nuclear extract (25
µg) from Hela cells Affinity purified Rabbit anti-Mre11 (ab3621) used at 1:4,000 dilution
Protocols
References
This product has been referenced in:
- Hematulin A et al. NBS1 is required for IGF-1 induced cellular proliferation through the Ras/Raf/MEK/ERK cascade. Cell Signal 20:2276-2285 (2008). Read more (PubMed: 18793719) »
