Overview

  • Product name

    Anti-Mre11 antibody - C-terminal
    See all Mre11 primary antibodies
  • Description

    Rabbit polyclonal to Mre11 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human Mre11 (C terminal). The exact sequence is proprietary.
    Database link: P49959

  • Positive control

    • WB: HeLa whole cell extract; IMR32 whole cell lysate. ICC/IF: SK-N-SH cells. IP: HeLa whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab227452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 81 kDa.

Target

  • Function

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
  • Involvement in disease

    Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
  • Sequence similarities

    Belongs to the MRE11/RAD32 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links

  • Alternative names

    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 homolog double strand break repair nuclease antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

Images

  • All lanes : Anti-Mre11 antibody - C-terminal (ab227452) at 1/1000 dilution

    Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : Mre11 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 81 kDa



    7.5% SDS-PAGE gel.

  • Anti-Mre11 antibody - C-terminal (ab227452) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 81 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Mre11 (green) using ab227452 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Mre11 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab227452. Western blot was performed from the immunoprecipitate using ab227452. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HeLa whole cell extract.

    Lane 2: Control IgG IP in HeLa whole cell extract.

    Lane 3: ab227452 IP in HeLa whole cell extract.

References

ab227452 has not yet been referenced specifically in any publications.

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