Key features and details
- Rabbit polyclonal to Mre11 - C-terminal
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Mre11 antibody - C-terminal
See all Mre11 primary antibodies
DescriptionRabbit polyclonal to Mre11 - C-terminal
Tested applicationsSuitable for: ICC/IF, IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment within Human Mre11 (C terminal). The exact sequence is proprietary.
Database link: P49959
- WB: HeLa whole cell extract; IMR32 whole cell lysate. ICC/IF: SK-N-SH cells. IP: HeLa whole cell extract.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|IP||1/100 - 1/500.|
|WB||1/500 - 1/3000. Predicted molecular weight: 81 kDa.|
FunctionComponent of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
Involvement in diseaseDefects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.
Sequence similaritiesBelongs to the MRE11/RAD32 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
- Information by UniProt
- AT like disease antibody
- Ataxia telangiectasia disorder like antibody
- ATLD antibody
All lanes : Anti-Mre11 antibody - C-terminal (ab227452) at 1/1000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Mre11 shRNA transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 81 kDa
7.5% SDS-PAGE gel.
Anti-Mre11 antibody - C-terminal (ab227452) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 81 kDa
7.5% SDS-PAGE gel.
4% paraformaldehyde-fixed SK-N-SH (human neuroblastoma cell line) cells stained for Mre11 (green) using ab227452 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Mre11 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab227452. Western blot was performed from the immunoprecipitate using ab227452. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract.
Lane 2: Control IgG IP in HeLa whole cell extract.
Lane 3: ab227452 IP in HeLa whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227452 has not yet been referenced specifically in any publications.