Recombinant
RabMAb

Recombinant Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)

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Overview

  • Product name

    Anti-Mre11 antibody [EPR21027] - ChIP Grade
    See all Mre11 primary antibodies

  • Description

    Rabbit monoclonal [EPR21027] to Mre11 - ChIP Grade

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ChIPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Mre11 aa 1-400. The exact sequence is proprietary.
    Database link: P49959

  • Positive control

    • WB: Human fetal kidney, mouse and rat brain tissue lysates; K562, Jurkat, HEK-293T, D3, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human colon, human endometrial carcinoma, mouse testis and rat colon tissues. FC: K562 cells. ChIP: HeLa whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab208020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/500.
ChIP Use at an assay dependent concentration.

5μg

Target

  • Function

    Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

  • Involvement in disease

    Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course.

  • Sequence similarities

    Belongs to the MRE11/RAD32 family.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AT like disease antibody
    • Ataxia telangiectasia disorder like antibody
    • ATLD antibody
    • DNA recombination and repair protein antibody
    • Double strand break repair protein MRE11A antibody
    • Double-strand break repair protein MRE11A antibody
    • endo/exonuclease Mre11 antibody
    • HNGS1 antibody
    • meiotic recombination (S. cerevisiae) 11 homolog A antibody
    • Meiotic recombination 11 homolog 1 antibody
    • meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • Meiotic recombination 11 homolog A antibody
    • MmMRE11A antibody
    • Mre 11 antibody
    • MRE 11a antibody
    • MRE 11b antibody
    • MRE11 homolog 1 antibody
    • MRE11 homolog A antibody
    • MRE11 homolog double strand break repair nuclease antibody
    • MRE11 meiotic recombination 11 homolog A (S. cerevisiae) antibody
    • MRE11 meiotic recombination 11 homolog A antibody
    • MRE11_HUMAN antibody
    • MRE11A antibody
    • MRE11b antibody
    • OTTHUMP00000236830 antibody
    • OTTHUMP00000236831 antibody
    • OTTHUMP00000236832 antibody
    • OTTHUMP00000236833 antibody
    see all

Images

  • Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/1000 dilution + Human fetal kidney tissue lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 81 kDa
    Observed band size: 81 kDa



    Blocking/Dilution: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    All lanes : Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/5000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : D3 (mouse embryo stem cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 81 kDa
    Observed band size: 81 kDa



    Blocking/Dilution: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Western blot - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    All lanes : Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 5 : Mouse brain tissue lysate
    Lane 6 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 81 kDa
    Observed band size: 81 kDa



    Blocking/Dilution: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in human colon tissue is observed (PMID: 25447316). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)

    Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in human endometrial carcinoma is observed (PMID: 24927325). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in mouse testis is observed (PMID: 10508516). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Mre11 with ab208020 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in rat colon tissue is observed (PMID: 28357369). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Flow Cytometry - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    Flow Cytometry - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (Human human chronic myelogenous leukemia cell line from bone marrow) cell line labeling Mre11 with ab208020 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • ChIP - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)
    ChIP - Anti-Mre11 antibody [EPR21027] - ChIP Grade (ab208020)

    Chromatin was prepared from non-treated HeLa cells or HeLa cells treated with 1% Methyl methanesulfonate for 1 hour according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 5 µg of ab208020 (blue), and 20 µL of protein A/G sepharose beads slurry (10 µL of sepharose A beads + 10 µL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

References

ab208020 has not yet been referenced specifically in any publications.

Publishing research using ab208020? Please let us know so that we can cite the reference in this datasheet.

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