Overview

  • Product name

    Anti-MRF-1 antibody
  • Description

    Rabbit polyclonal to MRF-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MRF-1.
    Database link: O75570

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human liver cancer tissue.
  • General notes

    Previously labelled as MTRF1.

Properties

Applications

Our Abpromise guarantee covers the use of ab222827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53,55 kDa).

Target

  • Function

    Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA.
  • Sequence similarities

    Belongs to the prokaryotic/mitochondrial release factor family.
  • Post-translational
    modifications

    Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • mitochondrial antibody
    • MRF-1 antibody
    • MRF1 antibody
    • MtRF-1 antibody
    • MTRF1 antibody
    • Peptide chain release factor 1 antibody
    • Peptide chain release factor 1, mitochondrial antibody
    • RF1M_HUMAN antibody
    see all

Images

  • Anti-MRF-1 antibody (ab222827) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary
    Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 53,55 kDa
    Observed band size: 53 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded sections of human liver cancer tissue were stained for MRF-1 using ab222827 at 1/100 dilution in immunohistochemical analysis.

References

ab222827 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222827.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up