Key features and details
- Rabbit polyclonal to MRF-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MRF-1 antibody
DescriptionRabbit polyclonal to MRF-1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human MRF-1.
Database link: O75570
- WB: HeLa whole cell lysate. IHC-P: Human liver cancer tissue.
Previously labelled as MTRF1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab222827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53,55 kDa).|
FunctionMitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain non-cognate termination stop codons AGG and AGA.
Sequence similaritiesBelongs to the prokaryotic/mitochondrial release factor family.
modificationsMethylation of glutamine in the GGQ triplet is conserved from bacteria to mammals.
- Information by UniProt
- mitochondrial antibody
- MRF-1 antibody
- MRF1 antibody
Anti-MRF-1 antibody (ab222827) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 53,55 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
Paraffin-embedded sections of human liver cancer tissue were stained for MRF-1 using ab222827 at 1/100 dilution in immunohistochemical analysis.
ab222827 has not yet been referenced specifically in any publications.