Key features and details
- Rabbit polyclonal to MRF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-MRF antibody
See all MRF primary antibodies
DescriptionRabbit polyclonal to MRF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- Rat brain tissue; mouse brain lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab213681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes.
Tissue specificityExpressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
Sequence similaritiesBelongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.
DomainA transmembrane region is predicted by sequence analysis tools. However, the protein is nuclear and probably acts as a transcription factor. It is therefore unclear whether the predicted transmembrane region is a hydrophobic region that does not insert into membranes or whether it is a real transmembrane region that may be cleaved when acting as transcription regulator.
Cellular localizationMembrane. Nucleus.
- Information by UniProt
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling MRF with ab213681 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Anti-MRF antibody (ab213681) at 1/200 dilution + mouse brain lysate
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution
Predicted band size: 124 kDa
ab213681 has been referenced in 1 publication.
- Wang CY et al. BRCA1/BRCA2-containing complex subunit 3 controls oligodendrocyte differentiation by dynamically regulating lysine 63-linked ubiquitination. Glia 67:1775-1792 (2019). PubMed: 31184779