Overview

  • Product name

  • Description

    Rabbit polyclonal to MRF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human MRF aa 465-515 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    NLPPEQ VTKVTVGRLH FSETTANNMR KKGKPNPDQR YFMLVVALQA HAQNQ


    Database link: Q9Y2G1

  • Positive control

    • Rat brain tissue; mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab213681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes.
  • Tissue specificity

    Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
  • Sequence similarities

    Belongs to the MRF family.
    Contains 1 NDT80 DNA-binding domain.
  • Domain

    A transmembrane region is predicted by sequence analysis tools. However, the protein is nuclear and probably acts as a transcription factor. It is therefore unclear whether the predicted transmembrane region is a hydrophobic region that does not insert into membranes or whether it is a real transmembrane region that may be cleaved when acting as transcription regulator.
  • Cellular localization

    Membrane. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C11orf9 antibody
    • Chromosome 11 open reading frame 9 antibody
    • GM1804 antibody
    • GM98 antibody
    • KIAA0954 antibody
    • MGC10781 antibody
    • MRF antibody
    • MRF_HUMAN antibody
    • Myelin gene regulatory factor antibody
    • Ndt80 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling MRF with ab213681 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • Anti-MRF antibody (ab213681) at 1/200 dilution + mouse brain lysate

    Secondary
    Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

    Predicted band size: 124 kDa



    6% gel

References

ab213681 has not yet been referenced specifically in any publications.

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