Key features and details
- Rabbit polyclonal to MRF - Oligodendrocyte Marker
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-MRF antibody - Oligodendrocyte Marker
See all MRF primary antibodies
DescriptionRabbit polyclonal to MRF - Oligodendrocyte Marker
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Rabbit
Synthetic peptide corresponding to Human MRF aa 1100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Mouse tissue lysates: Brain day 0; Embryonic brain E14; Embryonic brain E16.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).|
FunctionTranscription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes.
Tissue specificityExpressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
Sequence similaritiesBelongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.
DomainA transmembrane region is predicted by sequence analysis tools. However, the protein is nuclear and probably acts as a transcription factor. It is therefore unclear whether the predicted transmembrane region is a hydrophobic region that does not insert into membranes or whether it is a real transmembrane region that may be cleaved when acting as transcription regulator.
Cellular localizationMembrane. Nucleus.
- Information by UniProt
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All lanes : Anti-MRF antibody - Oligodendrocyte Marker (ab85464) at 1/250 dilution
Lane 1 :
Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 2 : E14 Mouse Embryo Brain Tissue Lysate
Lane 3 : E16 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 123 kDa
Observed band size: 123 kDa
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab85464 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab85464 has not yet been referenced specifically in any publications.