Product nameAnti-MRF antibody - Oligodendrocyte Marker
See all MRF primary antibodies
DescriptionRabbit polyclonal to MRF - Oligodendrocyte Marker
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Rabbit
Synthetic peptide corresponding to Human MRF aa 1100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Mouse tissue lysates: Brain day 0; Embryonic brain E14; Embryonic brain E16.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).|
FunctionTranscription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes.
Tissue specificityExpressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
Sequence similaritiesBelongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.
DomainA transmembrane region is predicted by sequence analysis tools. However, the protein is nuclear and probably acts as a transcription factor. It is therefore unclear whether the predicted transmembrane region is a hydrophobic region that does not insert into membranes or whether it is a real transmembrane region that may be cleaved when acting as transcription regulator.
Cellular localizationMembrane. Nucleus.
- Information by UniProt
- C11orf9 antibody
- Chromosome 11 open reading frame 9 antibody
- GM1804 antibody
All lanes : Anti-MRF antibody - Oligodendrocyte Marker (ab85464) at 1/250 dilution
Lane 1 :
Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 2 : E14 Mouse Embryo Brain Tissue Lysate
Lane 3 : E16 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 123 kDa
Observed band size: 123 kDa
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab85464 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab85464 has not yet been referenced specifically in any publications.