Overview

  • Product name

    Anti-MRF antibody - Oligodendrocyte Marker
    See all MRF primary antibodies
  • Description

    Rabbit polyclonal to MRF - Oligodendrocyte Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit
  • Immunogen

    Synthetic peptide corresponding to Human MRF aa 1100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab169733)

  • Positive control

    • This antibody gave a positive signal in the following Mouse tissue lysates: Brain day 0; Embryonic brain E14; Embryonic brain E16.

Properties

Applications

Our Abpromise guarantee covers the use of ab85464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 123 kDa (predicted molecular weight: 123 kDa).

Target

  • Function

    Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes.
  • Tissue specificity

    Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina.
  • Sequence similarities

    Belongs to the MRF family.
    Contains 1 NDT80 DNA-binding domain.
  • Domain

    A transmembrane region is predicted by sequence analysis tools. However, the protein is nuclear and probably acts as a transcription factor. It is therefore unclear whether the predicted transmembrane region is a hydrophobic region that does not insert into membranes or whether it is a real transmembrane region that may be cleaved when acting as transcription regulator.
  • Cellular localization

    Membrane. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C11orf9 antibody
    • Chromosome 11 open reading frame 9 antibody
    • GM1804 antibody
    • GM98 antibody
    • KIAA0954 antibody
    • MGC10781 antibody
    • MRF antibody
    • MRF_HUMAN antibody
    • Myelin gene regulatory factor antibody
    • Ndt80 antibody
    see all

Images

  • All lanes : Anti-MRF antibody - Oligodendrocyte Marker (ab85464) at 1/250 dilution

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 3 : E16 Ms Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 123 kDa
    Observed band size: 123 kDa
    Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab85464 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab85464 has not yet been referenced specifically in any publications.

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