Anti-MRG15 antibody (ab183663)
Key features and details
- Rabbit polyclonal to MRG15
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MRG15 antibody
See all MRG15 primary antibodies -
Description
Rabbit polyclonal to MRG15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Rhesus monkey
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Immunogen
Recombinant fragment within Human MRG15 aa 96-323. The exact sequence is proprietary.
Database link: Q9UBU8 -
Positive control
- Huh7, Hep3B and mouse liver whole cell lysate/extract; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab183663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 41 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci. -
Sequence similarities
Belongs to the MRG family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10933 Human
- Entrez Gene: 21761 Mouse
- Entrez Gene: 627352 Mouse
- Entrez Gene: 100340614 Rabbit
- Entrez Gene: 300891 Rat
- Entrez Gene: 711357 Rhesus monkey
- Omim: 607303 Human
- SwissProt: Q9UBU8 Human
see all -
Alternative names
- CG6363 antibody
- Eaf3 antibody
- Esa1p associated factor 3 homolog antibody
see all
Images
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Western blot - Anti-MRG15 antibody (ab183663)All lanes : Anti-MRG15 antibody (ab183663) at 1/500 dilution
Lane 1 : Huh7 whole cell lysate/extract
Lane 2 : Hep3B whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa10 % SDS-PAGE
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Western blot - Anti-MRG15 antibody (ab183663)Anti-MRG15 antibody (ab183663) at 1/500 dilution + Mouse liver lysate/extract at 50 µg
Predicted band size: 41 kDa10 % SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-MRG15 antibody (ab183663)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MRG15 with ab183663 at 1/1000 dilution (green). The slide on the right is counterstained with Hoechst 33342 (blue).
Protocols
References (1)
ab183663 has been referenced in 1 publication.
- Charoy C et al. Genetic specification of left-right asymmetry in the diaphragm muscles and their motor innervation. Elife 6:N/A (2017). WB ; Mouse . PubMed: 28639940
