Key features and details
- Rabbit polyclonal to MRG15
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-MRG15 antibody
See all MRG15 primary antibodies
DescriptionRabbit polyclonal to MRG15
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Rhesus monkey
Recombinant fragment within Human MRG15 aa 96-323. The exact sequence is proprietary.
Database link: Q9UBU8
- Huh7, Hep3B and mouse liver whole cell lysate/extract; HeLa cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 41 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionComponent of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci.
Sequence similaritiesBelongs to the MRG family.
- Information by UniProt
- CG6363 antibody
- Eaf3 antibody
- Esa1p associated factor 3 homolog antibody
All lanes : Anti-MRG15 antibody (ab183663) at 1/500 dilution
Lane 1 : Huh7 whole cell lysate/extract
Lane 2 : Hep3B whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
10 % SDS-PAGE
Anti-MRG15 antibody (ab183663) at 1/500 dilution + Mouse liver lysate/extract at 50 µg
Predicted band size: 41 kDa
10 % SDS-PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MRG15 with ab183663 at 1/1000 dilution (green). The slide on the right is counterstained with Hoechst 33342 (blue).
ab183663 has been referenced in 1 publication.
- Charoy C et al. Genetic specification of left-right asymmetry in the diaphragm muscles and their motor innervation. Elife 6:N/A (2017). WB ; Mouse . PubMed: 28639940