Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by immunogen affinity chromatography.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab15700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).Can be blocked with Human MRG15 peptide (ab23259).|
FunctionComponent of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones.
Sequence similaritiesBelongs to the MRG family.
- Information by UniProt
- KIAA0026 antibody
- MO4L2_HUMAN antibody
- MORF related gene X protein antibody
Anti-MRGX antibody (ab15700) at 0.25 µg/ml + Cell lysates prepared from A431 cells at 35 µg
Predicted band size: 33 kDa
ab15700 staining (0.2
g/ml) of A431 lysate (RIPA buffer, 35 µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. µ
Primary incubated fro 1 hour.
ab15700 has not yet been referenced specifically in any publications.