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    mrgx-antibody-epr97752-ab178405.pdf

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RecombinantRabMAb

Recombinant Anti-MRGX antibody [EPR9775(2)] (ab178405)

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Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)
  • Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)
  • Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)
  • Anti-MRGX antibody [EPR9775(2)] (ab178405)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9775(2)] to MRGX
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-MRGX antibody [EPR9775(2)]
    See all MRGX primary antibodies
  • Description

    Rabbit monoclonal [EPR9775(2)] to MRGX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, HeLa, A431 and U87-MG cell lysates; Human ovarian carcinoma and breast tissues; HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9775(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-MRGX antibody [EPR9775(2)] - BSA and Azide free (ab250034)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A-431 whole cell lysate (ab7909)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human MRGX protein (ab172166)
  • Related Products

    • Recombinant Human MRGX protein (ab105615)
    • Recombinant Human MRGX protein (ab172166)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones.
  • Sequence similarities

    Belongs to the MRG family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q15014 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9643 Human
    • Entrez Gene: 56397 Mouse
    • Entrez Gene: 317413 Rat
    • Omim: 300409 Human
    • SwissProt: Q15014 Human
    • SwissProt: Q9R0Q4 Mouse
    • SwissProt: Q6QI89 Rat
    • Unigene: 326387 Human
    • Unigene: 27218 Mouse
    • Unigene: 6961 Rat
    see all
  • Alternative names

    • KIAA0026 antibody
    • MO4L2_HUMAN antibody
    • MORF related gene X protein antibody
    • MORF-related gene X protein antibody
    • Morf4l2 antibody
    • MORFL2 antibody
    • Mortality Factor 4 like 2 antibody
    • Mortality factor 4 like protein 2 antibody
    • Mortality factor 4-like protein 2 antibody
    • MRGX antibody
    • MSL3 2 protein antibody
    • Protein MSL3-2 antibody
    • Transcription factor like protein MRGX antibody
    • Transcription factor-like protein MRGX antibody
    see all

Images

  • Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    All lanes : Anti-MRGX antibody [EPR9775(2)] (ab178405) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling MRGX with ab178405 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling MRGX with ab178405 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)

    Immunofluorescent analysis of HeLa cells labeling MRGX with ab178405 at 1/50 dilution.

  • Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling MRGX immunoprecipitated using ab178405 at 1/10 dilution.

  • Anti-MRGX antibody [EPR9775(2)] (ab178405)
    Anti-MRGX antibody [EPR9775(2)] (ab178405)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab178405? Please let us know so that we can cite the reference in this datasheet.

ab178405 has not yet been referenced specifically in any publications.

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