Recombinant Anti-MRGX antibody [EPR9775(2)] (ab178405)
![Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)](https://www.abcam.com/ps/products/178/ab178405/Images/ab178405-206348-anti-mrgx-antibody-epr97752-western-blot.jpg "Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9775(2)] to MRGX
- Suitable for: WB, ICC/IF, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MRGX antibody [EPR9775(2)]
See all MRGX primary antibodies -
Description
Rabbit monoclonal [EPR9775(2)] to MRGX -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- K562, HeLa, A431 and U87-MG cell lysates; Human ovarian carcinoma and breast tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9775(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
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| ICC/IF |
1/50 - 1/100.
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| IP |
1/10 - 1/100.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). |
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ICC/IF
1/50 - 1/100. |
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IP
1/10 - 1/100. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones. -
Sequence similarities
Belongs to the MRG family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9643 Human
- Entrez Gene: 56397 Mouse
- Entrez Gene: 317413 Rat
- Omim: 300409 Human
- SwissProt: Q15014 Human
- SwissProt: Q9R0Q4 Mouse
- SwissProt: Q6QI89 Rat
- Unigene: 326387 Human
see all -
Alternative names
- KIAA0026 antibody
- MO4L2_HUMAN antibody
- MORF related gene X protein antibody
see all
Images
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Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)All lanes : Anti-MRGX antibody [EPR9775(2)] (ab178405) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A431 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 32 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)](https://www.abcam.com/ps/products/178/ab178405/Images/ab178405-206347-anti-mrgx-antibody-epr97752-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling MRGX with ab178405 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)](https://www.abcam.com/ps/products/178/ab178405/Images/ab178405-206346-anti-mrgx-antibody-epr97752-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling MRGX with ab178405 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)](https://www.abcam.com/ps/products/178/ab178405/Images/ab178405-206345-anti-mrgx-antibody-epr97752-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)Immunofluorescent analysis of HeLa cells labeling MRGX with ab178405 at 1/50 dilution.
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![Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)](https://www.abcam.com/ps/products/178/ab178405/Images/ab178405-206344-anti-mrgx-antibody-epr97752-immunoprecipitation.jpg)
Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling MRGX immunoprecipitated using ab178405 at 1/10 dilution.
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![Anti-MRGX antibody [EPR9775(2)] (ab178405)](https://www.abcam.com/ps/products/178/ab178405/Images/ab178405-6-benefits-of-recombinant-antibodies.png)
Anti-MRGX antibody [EPR9775(2)] (ab178405)
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab178405 has not yet been referenced specifically in any publications.
