Recombinant
RabMAb

Recombinant Anti-MRGX antibody [EPR9775(2)] (ab178405)

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Overview

  • Product name

    Anti-MRGX antibody [EPR9775(2)]
    See all MRGX primary antibodies

  • Description

    Rabbit monoclonal [EPR9775(2)] to MRGX

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    within Human MRGX aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15014

  • Positive control

    • K562, HeLa, A431 and U87-MG cell lysates; Human ovarian carcinoma and breast tissues; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab178405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the MSIN3A complex which acts to repress transcription by deacetylation of nucleosomal histones.

    • Sequence similarities

      Belongs to the MRG family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • KIAA0026 antibody
      • MO4L2_HUMAN antibody
      • MORF related gene X protein antibody
      • MORF-related gene X protein antibody
      • Morf4l2 antibody
      • MORFL2 antibody
      • Mortality Factor 4 like 2 antibody
      • Mortality factor 4 like protein 2 antibody
      • Mortality factor 4-like protein 2 antibody
      • MRGX antibody
      • MSL3 2 protein antibody
      • Protein MSL3-2 antibody
      • Transcription factor like protein MRGX antibody
      • Transcription factor-like protein MRGX antibody
      see all

    Images

    • Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)
      Western blot - Anti-MRGX antibody [EPR9775(2)] (ab178405)
      All lanes : Anti-MRGX antibody [EPR9775(2)] (ab178405) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 32 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast tissue labeling MRGX with ab178405 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRGX antibody [EPR9775(2)] (ab178405)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling MRGX with ab178405 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)
      Immunocytochemistry/ Immunofluorescence - Anti-MRGX antibody [EPR9775(2)] (ab178405)

      Immunofluorescent analysis of HeLa cells labeling MRGX with ab178405 at 1/50 dilution.

    • Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)
      Immunoprecipitation - Anti-MRGX antibody [EPR9775(2)] (ab178405)

      Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling MRGX immunoprecipitated using ab178405 at 1/10 dilution.

    References

    ab178405 has not yet been referenced specifically in any publications.

    Publishing research using ab178405? Please let us know so that we can cite the reference in this datasheet.

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