Key features and details
- Rabbit polyclonal to MRGX1
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MRGX1 antibody
DescriptionRabbit polyclonal to MRGX1
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide derived from internal sequence of human GPCR MRGX1.
- Extracts from MCF-7 cells. HepG2 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab77519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 36 kDa.|
|ICC/IF||1/500 - 1/1000.|
RelevanceMRGX1 is an opiod receptor that has been reported exclusively in dorsal root ganglion. MRGX1 is probably involved in the function of nociceptive neurons and may regulate nociceptor function and/or development, including the sensation or modulation of pain. It is potently activated by enkephalins including BAM22 (bovine adrenal medulla peptide 22) and BAM (8-22). BAM22 is the most potent compound and evoked a large and dose-dependent release of intracellular calcium in stably transfected cells.
Cellular localizationCell Membrane; Multi-pass membrane protein.
- GPCR antibody
- MAS related gene MRGX1 antibody
- MAS related GPR member X1 antibody
All lanes : Anti-MRGX1 antibody (ab77519) at 1/500 dilution
Lane 1 : Extracts from MCF-7 cells
Lane 2 : Extracts from MCF-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Immunofluorescence analysis of GPCR MRGX1 in HepG2 cells using ab77519, at 1/500 dilution, in the absence or presence of immunising peptide
ab77519 has not yet been referenced specifically in any publications.