Key features and details
- Rabbit polyclonal to MRI
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MRI antibody
See all MRI primary antibodies
DescriptionRabbit polyclonal to MRI
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human duodenum, cerebral cortex and prostate tissue. WB: MRI overexpression HEK-293T lysate. ICC/IF: U-251 MG cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay act as a regulator of proteasome.
- Information by UniProt
- C7orf49 antibody
- Chromosome 7 open reading frame 49 antibody
- Modulator of retrovirus infection homolog antibody
PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling MRI using ab246926 at 4 µg/ml (green) in ICC/IF.
All lanes : Anti-MRI antibody (ab246926) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : MRI overexpression HEK-293T lysate
Formalin-fixed, paraffin-embedded human duodenum tissue stained for MRI with ab246926 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human prostate tissue stained for MRI with ab246926 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for MRI with ab246926 at a 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human pancreas tissue shows absence of immunoreactivity for MRI when incubated with ab246296 at a 1/50 dilution (negative control).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246926 has not yet been referenced specifically in any publications.