Product nameAnti-MRP1 antibody
See all MRP1 primary antibodies
DescriptionRabbit polyclonal to MRP1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Monkey
Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human MRP1.
- This antibody gave a positive signal in A549 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 171 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
Tissue specificityLung, testis and peripheral blood mononuclear cells.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
Cellular localizationCell membrane.
- Information by UniProt
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
Anti-MRP1 antibody (ab99531) at 1 µg/ml + A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 171 kDa
Exposure time: 8 minutes
MRP1 contains a number of potential glycosylation sites (SwissProt) which may explain the fuzzy appearance to the band observed at 171 kDa.
ICC/IF image of ab99531 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99531, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Li S et al. Down-regulation of miR-210-3p encourages chemotherapy resistance of renal cell carcinoma via modulating ABCC1. Cell Biosci 8:9 (2018). Read more (PubMed: 29445446) »
- Shi L et al. Up-regulation of miR-146a increases the sensitivity of non-small cell lung cancer to DDP by downregulating cyclin J. BMC Cancer 17:138 (2017). WB . Read more (PubMed: 28202053) »