Name: Anti-MRP1 antibody [EPR21061]
Brand: RabMAb
SKU: ab230948

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Primary

Secondary

Protein

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Primary - Rabbit Anti-MRP1 antibody [EPR21061] (ab230948)
Flow Cyt, IHC-P, WB

Secondary - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Fr

Protein - Recombinant Human MRP1 protein (His tag) (ab235016)
SDS-PAGE

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Product name

Anti-MRP1 antibody [EPR21061]
See all MRP1 primary antibodies
Description

Rabbit monoclonal [EPR21061] to MRP1
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human MRP1 aa 1500 to the C-terminus. The exact sequence is proprietary.
Database link: P33527
Positive control
- WB: HeLa, A549, BxPC-3, SK-OV-3 and HCT 116 whole cell lysate. IHC-P: Human colon cancer, esophagus cancer and lung cancer tissue. Flow Cytometry: HeLa cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21061
Isotype

IgG
Research areas

Alternative Versions
- Anti-MRP1 antibody [EPR21061] - BSA and Azide free (ab234104)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Immunohistochemistry reagents
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human MRP1 protein (His tag) (ab235016)

Our Abpromise guarantee covers the use of ab230948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		1/50.
IHC-P		1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Predicted molecular weight: 171 kDa.

Function

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
Tissue specificity

Lung, testis and peripheral blood mononuclear cells.
Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P33527 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4363 Human
- Omim: 158343 Human
- SwissProt: P33527 Human
- Unigene: 391464 Human
Alternative names
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
- ABCC antibody
- Abcc1 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
- ATP binding cassette sub-family C member 1 antibody
- ATP binding cassette subfamily C member 1 antibody
- ATP binding cassette transporter variant ABCC1delta ex13 antibody
- ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
- ATP binding cassette transporter variant ABCC1delta ex25 antibody
- ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
- ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
- ATP-binding cassette sub-family C member 1 antibody
- DKFZp686N04233 antibody
- DKFZp781G125 antibody
- GS X antibody
- GSX antibody
- Leukotriene C(4) transporter antibody
- LTC4 transporter antibody
- MRP 1 antibody
- MRP antibody
- MRP1 antibody
- MRP1_HUMAN antibody
- Multidrug resistance associated protein 1 antibody
- Multidrug resistance protein antibody
- Multidrug resistance-associated protein 1 antibody
- Multiple drug resistance associated protein antibody
- Multiple drug resistance protein 1 antibody
see all

Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)

All lanes : Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate
Lane 5 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 171 kDa

Exposure time: 3 minutes

Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.
Flow Cytometry - Anti-MRP1 antibody [EPR21061] (ab230948)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling MRP1 with ab230948 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

Immunohistochemical analysis of paraffin-embedded human esophagus cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous staining in human esophageal cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:26870278) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

Immunohistochemical analysis of paraffin-embedded human lung cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human lung cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

Immunohistochemical analysis of paraffin-embedded human colon cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human colon cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Datasheet

ab230948 has not yet been referenced specifically in any publications.

Publishing research using ab230948? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Recombinant Anti-MRP1 antibody [EPR21061] (ab230948)

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Immunohistochemistry reagents

Isotype control

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Datasheets and documents

References

Customer reviews and Q&As