Recombinant Anti-MRP1 antibody [EPR21061] (ab230948)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21061] to MRP1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MRP1 antibody [EPR21061]
See all MRP1 primary antibodies -
Description
Rabbit monoclonal [EPR21061] to MRP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, A549, BxPC-3, SK-OV-3 and HCT 116 whole cell lysate. IHC-P: Human colon cancer, esophagus cancer and lung cancer tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21061 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 171 kDa.
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Notes |
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IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 171 kDa. |
Target
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Function
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. -
Tissue specificity
Lung, testis and peripheral blood mononuclear cells. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4363 Human
- Omim: 158343 Human
- SwissProt: P33527 Human
- Unigene: 391464 Human
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Alternative names
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
see all
Images
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All lanes : Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : ABCC1 knockout A549 cell lysate
Lane 3 : Wild-type HeLa cell lysate
Lane 4 : ABCC1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab230948 observed at 170 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab230948 was shown to react with MRP1 in wild-type HeLa cells in Western blot with loss of signal observed in ABCC1 knockout cell line (ABCC1 knockout cell lysate). Wild-type HeLa and ABCC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab230948 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
Immunohistochemical analysis of paraffin-embedded human esophagus cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous staining in human esophageal cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:26870278) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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All lanes : Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate
Lane 5 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 171 kDa
Exposure time: 3 minutesBlocking/ Dilution buffer and concentration: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
Immunohistochemical analysis of paraffin-embedded human lung cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human lung cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
Immunohistochemical analysis of paraffin-embedded human colon cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human colon cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab230948 has been referenced in 1 publication.
- Xu X et al. Exosome-transferred hsa_circ_0014235 promotes DDP chemoresistance and deteriorates the development of non-small cell lung cancer by mediating the miR-520a-5p/CDK4 pathway. Cancer Cell Int 20:552 (2020). PubMed: 33292236