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  1. Link

    mrp1-antibody-epr21061-ab230948.pdf

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Signal Transduction Metabolism Plasma Membrane Channels
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MRP1 antibody [EPR21061] (ab230948)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (1)

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Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
  • Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
  • Anti-MRP1 antibody [EPR21061] (ab230948)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21061] to MRP1
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Human ABCC1 (MRP1) knockout HeLa cell line (ab265256)

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Overview

  • Product name

    Anti-MRP1 antibody [EPR21061]
    See all MRP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR21061] to MRP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A549, BxPC-3, SK-OV-3 and HCT 116 whole cell lysate. IHC-P: Human colon cancer, esophagus cancer and lung cancer tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21061
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Cancer
    • Drug resistance
    • MRP-related proteins
    • Cardiovascular
    • Atherosclerosis
    • Hypertension
    • Sodium/ion transport
    • Cardiovascular
    • Blood
    • Blood Pressure regulation
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-MRP1 antibody [EPR21061] - BSA and Azide free (ab234104)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunohistochemistry reagents

    • Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MRP1 protein (His tag) (ab235016)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab230948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 171 kDa.
Notes
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 171 kDa.

Target

  • Function

    Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • Tissue specificity

    Lung, testis and peripheral blood mononuclear cells.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P33527 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4363 Human
    • Omim: 158343 Human
    • SwissProt: P33527 Human
    • Unigene: 391464 Human
    • Alternative names

      • ABC 29 antibody
      • ABC29 antibody
      • ABCC 1 antibody
      • ABCC antibody
      • Abcc1 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
      • ATP binding cassette sub-family C member 1 antibody
      • ATP binding cassette subfamily C member 1 antibody
      • ATP binding cassette transporter variant ABCC1delta ex13 antibody
      • ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
      • ATP binding cassette transporter variant ABCC1delta ex25 antibody
      • ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
      • ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
      • ATP-binding cassette sub-family C member 1 antibody
      • DKFZp686N04233 antibody
      • DKFZp781G125 antibody
      • GS X antibody
      • GSX antibody
      • Leukotriene C(4) transporter antibody
      • LTC4 transporter antibody
      • MRP 1 antibody
      • MRP antibody
      • MRP1 antibody
      • MRP1_HUMAN antibody
      • Multidrug resistance associated protein 1 antibody
      • Multidrug resistance protein antibody
      • Multidrug resistance-associated protein 1 antibody
      • Multiple drug resistance associated protein antibody
      • Multiple drug resistance protein 1 antibody
      see all

    Images

    • Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)
      Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)
      All lanes : Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution

      Lane 1 : Wild-type A549 cell lysate
      Lane 2 : ABCC1 knockout A549 cell lysate
      Lane 3 : Wild-type HeLa cell lysate
      Lane 4 : ABCC1 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 171 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab230948 observed at 170 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

      ab230948 was shown to react with MRP1 in wild-type HeLa cells in Western blot with loss of signal observed in ABCC1 knockout cell line (ABCC1 knockout cell lysate). Wild-type HeLa and ABCC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab230948 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

      Immunohistochemical analysis of paraffin-embedded human esophagus cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous staining in human esophageal cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:26870278) is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)
      Western blot - Anti-MRP1 antibody [EPR21061] (ab230948)
      All lanes : Anti-MRP1 antibody [EPR21061] (ab230948) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate
      Lane 3 : BxPC-3 (human pancreas adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate
      Lane 5 : HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 171 kDa


      Exposure time: 3 minutes


      Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

      Immunohistochemical analysis of paraffin-embedded human lung cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human lung cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR21061] (ab230948)

      Immunohistochemical analysis of paraffin-embedded human colon cancer (B) and its adjacent noncancerous tissue (A) labeling MRP1 with ab230948 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Strong membranous and cytoplasmic staining in human colon cancer tissue (B) with weak staining in its adjacent noncancerous tissue (A) (PMID:23667609) is observed. Counter stained with Hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Anti-MRP1 antibody [EPR21061] (ab230948)
      Anti-MRP1 antibody [EPR21061] (ab230948)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (1)

    Publishing research using ab230948? Please let us know so that we can cite the reference in this datasheet.

    ab230948 has been referenced in 1 publication.

    • Xu X  et al. Exosome-transferred hsa_circ_0014235 promotes DDP chemoresistance and deteriorates the development of non-small cell lung cancer by mediating the miR-520a-5p/CDK4 pathway. Cancer Cell Int 20:552 (2020). PubMed: 33292236

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