Recombinant Anti-MRP1 antibody [EPR22841-78] (ab260038)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22841-78] to MRP1
- Suitable for: Flow Cyt (Intra), IHC-P, WB, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-MRP1 antibody [EPR22841-78]
See all MRP1 primary antibodies -
Description
Rabbit monoclonal [EPR22841-78] to MRP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, HepG2, K-562, Human lung cancer, Human lung, NIH/3T3, Mouse liver and Mouse stomach lysates. IHC-P: Human breast carcinoma, Human gastric adenocarcinoma, Human lung carcinoma and the paired adjacent lung and Mouse stomach tissues. Flow Cyt (intra): HeLa, A549 and NIH/3T3 cells. IP: A549 and NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22841-78 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab260038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/600.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 171 kDa).
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IP |
1/30.
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Notes |
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Flow Cyt (Intra)
1/600. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
WB
1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 171 kDa).
|
IP
1/30. |
Target
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Function
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. -
Tissue specificity
Lung, testis and peripheral blood mononuclear cells. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4363 Human
- Entrez Gene: 17250 Mouse
- Omim: 158343 Human
- SwissProt: P33527 Human
- SwissProt: O35379 Mouse
- Unigene: 391464 Human
- Unigene: 196634 Mouse
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Alternative names
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
see all
Images
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All lanes : Anti-MRP1 antibody [EPR22841-78] (ab260038) at 1/1000 dilution
Lanes 1-2 : A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 5 : Human lung cancer lysate at 20 µg
Secondary
Lanes 1 & 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution
Predicted band size: 171 kDa
Observed band size: 120-250 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 27634913).
Weakly expressing control: K-562, lung, liver, HEK293 (PMID: 18347152, 25880778,11059771, 29615646, 26853103).
Samples (except lane1) are non-boiled as boiling may cause protein aggregates. The Lane 1 lysate was boiled on 100? water.
Exposure time: 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on mouse stomach (PMID: 23667609). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Intracellular Flow Cytometry overlay histogram showing wild-type HeLa (green line) and ABCC1 knockout HeLa cells (ab265256). The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 22°C.) stained with ab260038 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab260038) (1x106 in 100µl at 0.2 µg/ml) for 30 min at 22°C.
Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line ABCC1 knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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All lanes : Anti-MRP1 antibody [EPR22841-78] (ab260038) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 4 : Human lung cancer lysate at 20 µg
Lane 5 : Human lung lysate at 20 µg
Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Lane 7 : Mouse liver lysate at 10 µg
Lane 8 : Mouse stomach lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 171 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 27634913).
Weakly expressing control: K-562, lung, liver, HEK293 (PMID: 18347152, 25880778,11059771, 29615646, 26853103).
All lysates were unboiled.
Exposure time: Lanes 1-3: 10 seconds; Lanes 4-8: 3 minutes.
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MRP1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab260038 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug
Lane 2: ab260038 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate, boiled 10ug
Lane 3: NIH/3T3 whole cell lysate
Lane 4: Rabbit monoclonal IgG (ab172730) instead of ab260038 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 75 seconds
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MRP1 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate with ab260038 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab260038 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate, boiled 10ug
Lane 3: A549 whole cell lysate
Lane 4: Rabbit monoclonal IgG (ab172730) instead of ab260038 in A549 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma and the paired adjacent lung tissue tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human lung carcinoma (panel A) and no staining on the paired adjacent lung tissue (panel B, PMID: 25880778). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human gastric adenocarcinoma. The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human breast carcinoma (PMID: 15517899). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling MRP1 with ab260038 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell, Left) / A549 (human lung carcinoma epithelial cell, Right) cells labelling MRP1 with ab260038 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: HEK-293 (PMID: 29615646, PMID:12235150).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab260038 has been referenced in 2 publications.
- Li D et al. MicroRNA-375 represses tumor angiogenesis and reverses resistance to sorafenib in hepatocarcinoma. Cancer Gene Ther 28:126-140 (2021). PubMed: 32616906
- Xu L et al. AITC induces MRP1 expression by protecting against CS/CSE-mediated DJ-1 protein degradation via activation of the DJ-1/Nrf2 axis. Korean J Physiol Pharmacol 24:481-492 (2020). PubMed: 33093270