Recombinant
RabMAb

Recombinant Anti-MRP1 antibody [EPR22841-78] (ab260038)

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Overview

  • Product name

    Anti-MRP1 antibody [EPR22841-78]
    See all MRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22841-78] to MRP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human MRP1 aa 1200 to the C-terminus. The exact sequence is proprietary.
    Database link: P33527

  • Positive control

    • WB: A549, HepG2, K-562, Human lung cancer, Human lung, NIH/3T3, Mouse liver and Mouse stomach lysates. IHC-P: Human breast carcinoma, Human gastric adenocarcinoma, Human lung carcinoma and the paired adjacent lung and Mouse stomach tissues. FC: A549 and NIH/3T3 cells. IP: A549 and NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab260038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

 

 
WB 1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 171 kDa).

 

 
Flow Cyt 1/600.
IP 1/30.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.

    • Tissue specificity

      Lung, testis and peripheral blood mononuclear cells.

    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • ABC 29 antibody
        • ABC29 antibody
        • ABCC 1 antibody
        • ABCC antibody
        • Abcc1 antibody
        • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
        • ATP binding cassette sub-family C member 1 antibody
        • ATP binding cassette subfamily C member 1 antibody
        • ATP binding cassette transporter variant ABCC1delta ex13 antibody
        • ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
        • ATP binding cassette transporter variant ABCC1delta ex25 antibody
        • ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
        • ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
        • ATP-binding cassette sub-family C member 1 antibody
        • DKFZp686N04233 antibody
        • DKFZp781G125 antibody
        • GS X antibody
        • GSX antibody
        • Leukotriene C(4) transporter antibody
        • LTC4 transporter antibody
        • MRP 1 antibody
        • MRP antibody
        • MRP1 antibody
        • MRP1_HUMAN antibody
        • Multidrug resistance associated protein 1 antibody
        • Multidrug resistance protein antibody
        • Multidrug resistance-associated protein 1 antibody
        • Multiple drug resistance associated protein antibody
        • Multiple drug resistance protein 1 antibody
        see all

      Images

      • Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        All lanes : Anti-MRP1 antibody [EPR22841-78] (ab260038) at 1/1000 dilution

        Lanes 1-2 : A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
        Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
        Lane 4 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
        Lane 5 : Human lung cancer lysate at 20 µg

        Secondary
        Lanes 1 & 4-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
        Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution

        Predicted band size: 171 kDa
        Observed band size: 120-250 kDa
        why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        The expression profile observed is consistent with what has been described in the literature (PMID: 27634913).

        Weakly expressing control: K-562, lung, liver, HEK293 (PMID: 18347152, 25880778,11059771, 29615646, 26853103).

        Samples (except lane1) are non-boiled as boiling may cause protein aggregates. The Lane 1 lysate was boiled on 100? water.

        Exposure time: 3 minutes.

      • Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Western blot - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        All lanes : Anti-MRP1 antibody [EPR22841-78] (ab260038) at 1/1000 dilution

        Lane 1 : A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
        Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
        Lane 3 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
        Lane 4 : Human lung cancer lysate at 20 µg
        Lane 5 : Human lung lysate at 20 µg
        Lane 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
        Lane 7 : Mouse liver lysate at 10 µg
        Lane 8 : Mouse stomach lysate at 10 µg

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 171 kDa
        Observed band size: 190 kDa why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST.

        The expression profile observed is consistent with what has been described in the literature (PMID: 27634913). 

        Weakly expressing control: K-562, lung, liver, HEK293 (PMID: 18347152, 25880778,11059771, 29615646, 26853103). 

        All lysates were unboiled. 

        Exposure time: Lanes 1-3: 10 seconds; Lanes 4-8: 3 minutes.

      • Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038)

        MRP1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab260038 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

        Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug

        Lane 2: ab260038 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate, boiled 10ug

        Lane 3: NIH/3T3 whole cell lysate

        Lane 4: Rabbit monoclonal IgG (ab172730) instead of ab260038 in NIH/3T3 whole cell lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 75 seconds

         

      • Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Immunoprecipitation - Anti-MRP1 antibody [EPR22841-78] (ab260038)

        MRP1 was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) whole cell lysate  with ab260038 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260038 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

        Lane 1: A549 (human lung carcinoma epithelial cell) whole cell lysate 10ug

        Lane 2: ab260038 IP in A549 (human lung carcinoma epithelial cell) whole cell lysate, boiled 10ug

        Lane 3: A549 whole cell lysate

        Lane 4: Rabbit monoclonal IgG (ab172730) instead of ab260038 in A549 whole cell lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 3 seconds.

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)

        Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on mouse stomach (PMID: 23667609). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)

        Immunohistochemical analysis of paraffin-embedded human lung carcinoma and the paired adjacent lung tissue tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human lung carcinoma (panel A) and no staining on the paired adjacent lung tissue (panel B, PMID: 25880778). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)

        Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human gastric adenocarcinoma. The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [EPR22841-78] (ab260038)

        Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MRP1 with ab260038 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous and weakly cytoplasmic staining on human breast carcinoma (PMID: 15517899). The section was incubated with ab260038 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      • Flow Cytometry - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Flow Cytometry - Anti-MRP1 antibody [EPR22841-78] (ab260038)

        Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling MRP1 with ab260038 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      • Flow Cytometry - Anti-MRP1 antibody [EPR22841-78] (ab260038)
        Flow Cytometry - Anti-MRP1 antibody [EPR22841-78] (ab260038)

        Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell, Left) / A549 (human lung carcinoma epithelial cell, Right) cells labelling MRP1 with ab260038 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        Negative control: HEK-293 (PMID: 29615646，PMID:12235150).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References

      Publishing research using ab260038? Please let us know so that we can cite the reference in this datasheet.

      ab260038 has not yet been referenced specifically in any publications.

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