Overview

  • Product name
    Anti-MRP1 antibody [IU2H10]
    See all MRP1 primary antibodies
  • Description
    Mouse monoclonal [IU2H10] to MRP1
  • Host species
    Mouse
  • Specificity
    This antibody is specific for MRP1 protein.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCF, corresponding to amino acids 1-33 of Human MRP1

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab24102 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab32574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 172 kDa. There may be a lower MW band that is a degradation product.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • Tissue specificity
    Lung, testis and peripheral blood mononuclear cells.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 29 antibody
    • ABC29 antibody
    • ABCC 1 antibody
    • ABCC antibody
    • Abcc1 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
    • ATP binding cassette sub-family C member 1 antibody
    • ATP binding cassette subfamily C member 1 antibody
    • ATP binding cassette transporter variant ABCC1delta ex13 antibody
    • ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
    • ATP binding cassette transporter variant ABCC1delta ex25 antibody
    • ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
    • ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
    • ATP-binding cassette sub-family C member 1 antibody
    • DKFZp686N04233 antibody
    • DKFZp781G125 antibody
    • GS X antibody
    • GSX antibody
    • Leukotriene C(4) transporter antibody
    • LTC4 transporter antibody
    • MRP 1 antibody
    • MRP antibody
    • MRP1 antibody
    • MRP1_HUMAN antibody
    • Multidrug resistance associated protein 1 antibody
    • Multidrug resistance protein antibody
    • Multidrug resistance-associated protein 1 antibody
    • Multiple drug resistance associated protein antibody
    • Multiple drug resistance protein 1 antibody
    see all

Images

  • All lanes : Anti-MRP1 antibody [IU2H10] (ab32574)

    Lane 1 : Vector-transfected HEK293 cells
    Lane 2 : MRP1-transfected cells, prep1
    Lane 3 : MRP1-transfected cells, prep2 (the lower band is a degradation product)

    Predicted band size: 172 kDa

  • Ab32574 staining MRP1 in HeLa cells by Immunocytochemistry/Immunofluorescence (ICC/IF). Samples were incubated with primary antibody at 1:50 dilution against Dylight 488 (green). Alpha tubulin and nuclei were counterstained with Dylight 550 (red) and DAPI (blue) respectively.

References

This product has been referenced in:
  • Shang J  et al. Therapeutic Effects of Pretreatment with Tocovid on Oxidative Stress in Postischemic Mice Brain. J Stroke Cerebrovasc Dis 27:2096-2105 (2018). Read more (PubMed: 29793801) »
  • Schwiebs A  et al. Nuclear Translocation of SGPP-1 and Decrease of SGPL-1 Activity Contribute to Sphingolipid Rheostat Regulation of Inflammatory Dendritic Cells. Mediators Inflamm 2017:5187368 (2017). Read more (PubMed: 29375197) »

See all 17 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (brain endothelial cells)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
35 µg
Specification
brain endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

Submitted Dec 19 2017

Thank you for contacting us.

For ab108312, you could use human fetal liver (ab29890), JAR, 293T (ab95494), HepG2 (ab7900) or human placenta (ab29745) lysates as a positive control.

For ab32574, you could consider using MRP-1 trans...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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