Overview

  • Product name
    Anti-MRP1 antibody [IU2H10]
    See all MRP1 primary antibodies
  • Description
    Mouse monoclonal [IU2H10] to MRP1
  • Host species
    Mouse
  • Specificity
    This antibody is specific for MRP1 protein.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: MALRGFCSAD GSDPLWDWNV TWNTSNPDFT KCF, corresponding to amino acids 1-33 of Human MRP1

Properties

Applications

Our Abpromise guarantee covers the use of ab32574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 172 kDa. There may be a lower MW band that is a degradation product.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • Tissue specificity
    Lung, testis and peripheral blood mononuclear cells.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 29 antibody
    • ABC29 antibody
    • ABCC 1 antibody
    • ABCC antibody
    • Abcc1 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
    • ATP binding cassette sub-family C member 1 antibody
    • ATP binding cassette subfamily C member 1 antibody
    • ATP binding cassette transporter variant ABCC1delta ex13 antibody
    • ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
    • ATP binding cassette transporter variant ABCC1delta ex25 antibody
    • ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
    • ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
    • ATP-binding cassette sub-family C member 1 antibody
    • DKFZp686N04233 antibody
    • DKFZp781G125 antibody
    • GS X antibody
    • GSX antibody
    • Leukotriene C(4) transporter antibody
    • LTC4 transporter antibody
    • MRP 1 antibody
    • MRP antibody
    • MRP1 antibody
    • MRP1_HUMAN antibody
    • Multidrug resistance associated protein 1 antibody
    • Multidrug resistance protein antibody
    • Multidrug resistance-associated protein 1 antibody
    • Multiple drug resistance associated protein antibody
    • Multiple drug resistance protein 1 antibody
    see all

Images

  • All lanes : Anti-MRP1 antibody [IU2H10] (ab32574)

    Lane 1 : Vector-transfected HEK293 cells
    Lane 2 : MRP1-transfected cells, prep1
    Lane 3 : MRP1-transfected cells, prep2 (the lower band is a degradation product)

    Predicted band size: 172 kDa

  • Ab32574 staining MRP1 in HeLa cells by Immunocytochemistry/Immunofluorescence (ICC/IF). Samples were incubated with primary antibody at 1:50 dilution against Dylight 488 (green). Alpha tubulin and nuclei were counterstained with Dylight 550 (red) and DAPI (blue) respectively.

References

This product has been referenced in:
  • Shang J  et al. Therapeutic Effects of Pretreatment with Tocovid on Oxidative Stress in Postischemic Mice Brain. J Stroke Cerebrovasc Dis 27:2096-2105 (2018). Read more (PubMed: 29793801) »
  • Schwiebs A  et al. Nuclear Translocation of SGPP-1 and Decrease of SGPL-1 Activity Contribute to Sphingolipid Rheostat Regulation of Inflammatory Dendritic Cells. Mediators Inflamm 2017:5187368 (2017). Read more (PubMed: 29375197) »
See all 17 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Western blot
Sample
Pig Cell lysate - whole cell (brain endothelial cells)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
35 µg
Specification
brain endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Dec 19 2017

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