Anti-MRP1 antibody [MRPm5] (ab24102)

I'm assuming you're referring to a lysis buffer for sample preparation prior to western blot. If this is the case, since MRP1 is a membrane bound protein we recommend using RIPA buffer for lysis. Our 10X RIPA buffer ab156034 is through the link below:

https://www.abcam.com/10x-ripa-buffer-ab156034.html

Our sample preparation guide below is helpful if you have lysis buffer questions for other protein targets:

https://www.abcam.com/index.html?pageconfig=resource&rid=11379

Thank you for contacting us.

I do hope the suggestions made today shed some light upon the issue you are experiencing with ab24102 Anti-MRP1 antibody [MRPm5] and look forward to hear the results.

This product is covered by our Abpromise guarantee. If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, as you are using it according to specifications listed on our datasheet.

I have found some additional information about large protein transfer which may also be of help to you. The following information is from our Western Blot guide and may be found at: https://www.abcam.com/index.html?pageconfig=resource&rid=11404

Note on transfer of large and small proteins
The balance of SDS and methanol in the transfer buffer, protein size, and gel percentage can affect transfer efficiency. The following modifications will encourage efficient transfer.
Large proteins (>100 kD)
For large proteins, transfer out of the gel may be very slow, just as they run slowly within the gel during separation. If blotting a large protein, be sure to run your samples in a low-concentration gel, 8% or less. These will be very fragile, so handle carefully.
Large proteins will tend to precipitate in the gel, hindering transfer. Adding SDS to a final concentration of 0.1% in the transfer buffer will discourage this. Methanol tends to remove SDS from proteins, so reducing the methanol percentage to 10% or less will also guard against precipitation.
Lowering methanol in the transfer buffer also promotes swelling of the gel, allowing large proteins to transfer more easily.
Methanol is only necessary if using nitrocellulose. If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich.
Choose wet transfer overnight at 4°C instead of semi-dry transfer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.



Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102.
https://www.abcam.com/index.html?datasheet=29889 https://www.abcam.com/index.html?datasheet=29889.
MRP4 is barely detectable in liver and therefore I recommend to use ab30304 (Prostate (Human) Tissue Lysate - adult normal tissue) as positive control.
https://www.abcam.com/index.html?datasheet=30304 https://www.abcam.com/index.html?datasheet=30304.
With ab63907 we used ab29745 (Placenta (Human) Tissue Lysate - adult normal tissue) successfully as control.
https://www.abcam.com/index.html?datasheet=29745 https://www.abcam.com/index.html?datasheet=29745.
I hope this information is helpful and wish you good luck with your research.

Thank you for your enquiry and your interest in our products.

Here are a few antibodies against the following targets which have been tested and characterized for Flow cytometry. Please take a look at the on-line datasheets for further information about tested species/application/purity/concentration.

P-gp (MDR1)



ab3366: https://www.abcam.com/P-Glycoprotein-antibody-JSB-1-ab3366.html

ab3083: https://www.abcam.com/P-Glycoprotein-antibody-265-F4-ab3083.html

ab80594:https://www.abcam.com/P-Glycoprotein-antibody-F4-BSA-and-Azide-free-ab80594.html



MRP1:



ab24102: https://www.abcam.com/MRP1-antibody-MRPm5-ab24102.html



BCRP:



ab24115: https://www.abcam.com/BCRPABCG2-antibody-BXP-53-ab24115.html



If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for contacting us. We do have products that are conjugated to match your needs, Ab81424 Anti-CD44 antibody [MEM-263] is conjugated with APC. Ab58754 Anti-CD44 antibody [MEM-263] (Phycoerythrin). I've included links to each below: https://www.abcam.com/CD44-antibody-MEM-263-Allophycocyanin-ab81424.html https://www.abcam.com/CD44-antibody-MEM-263-Phycoerythrin-Extracellular-domain-ab58754.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam. We offer a number of PGP antibodies that are guaranteed to work in Flow Cytometry on dog cells. I would recommend https://www.abcam.com/CD44-antibody-IM7-ab119863.htmlor the FITC conjugated https://www.abcam.com/CD44-antibody-IM7-FITC-ab19622.htmlantibodies. I've pasted the link to the search results below: https://www.abcam.com/index.html?pageconfig=searchresults If you also require a MRP1 antibody that works in Flow Cytometry we offer ab24102 Anti-MRP1 antibody [MRPm5] ( https://www.abcam.com/MRP1-antibody-MRPm5-ab24102.html). This has not yet been tested in dog and would not therefore to covered by our Abpromise. However, because of the high homology between the dog protein and human protein that this was derived from we can offer you an Abreview discount. Under this plan if you buy ab24102 now, test it indog and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab24102 in dog. I will then send a discount code. This code must be issued before purchasing ab24102 so please wait for my reply before ordering. 2. Purchase ab24105 either by phone, fax, or online (www.abcam.com). 3. Test it in dog. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab24102 turns out to be unsuitable for dog, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the datasheets of these antibodies and select a product according to your requirements. The anti MDR2 products are ab71792; ab24108 and ab111209 Anti MRP1 products are ab24102; ab63987; ab99531; ab81903 To search different products please type target name in the search box provided on the Abcam homepage at www.abcam.com. After hitting the search button the system will give you the sorted product as per your target request. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

The only recommendation I have seen for a western blot positive control is a lysate from cells transfected with MRP1. We do not have such a lysate or a recombinant protein. As I mentioned in our phone call, endogenous sources include lung, testis, and peripheral mononuclear cells. I searched the literature for a cell line that might be a suitable positive control (we have many lysates) and found this article: Cloning and Characterization of the Murine and Rat mrp1 Promoter Regions Mol Pharmacol. 2003 Nov;64(5):1259-69. which mentions HL60/ADR and H69AR, which we do not have. The article may suggest other potential candidates. I hope this helps. If you have any other questions, please do not hesitate to contact us.