Product nameAnti-MRP1 antibody [MRPm5]
See all MRP1 primary antibodies
DescriptionMouse monoclonal [MRPm5] to MRP1
SpecificityMRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
Tested applicationsSuitable for: IHC-Fr, IHC-P, Flow Cyt, ICC/IF, WBmore details
Species reactivityReacts with: Human
Fusion protein corresponding to Human MRP1 aa 986-1204.
HVSALASNYWLSLWTDDPIVNGTQEHTKVRLSVYGALGISQGIAVFGYSM AVSIGGILASRCLHVDLLHSILRSPMSFFERTPSGNLVNRFSKELDTVDS MIPEVIKMFMGSLFNVIGACIVILLATPIAAIIIPPLGLIYFFVQRFYVA SSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIHQSDLKVDEN QKAYYPSIVANRWLAVRLE
Database link: P33527
EpitopeMRP1 antibody reacts with an internal epitope of MRP1.
- Flow Cyt: HL-60 cells. ICC/IF: Human ES cell derived hepatocytes.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS
Serum free tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab24102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval in citrate buffer (pH 6.0) for 10 minutes before commencing with IHC staining protocol.
|Flow Cyt||1/20 - 1/50.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/20 - 1/50.|
|WB||1/20 - 1/50. Predicted molecular weight: 172 kDa.|
FunctionMediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
Tissue specificityLung, testis and peripheral blood mononuclear cells.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
Cellular localizationCell membrane.
- Information by UniProt
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
ICC/IF image of Anti-MRP1 antibody (ab24102) stained human ES cell derived hepatocytes. The cells were fixed in methanol, blocked and permeabilized using 10% BSA in PBS/0.1% Tween, for 1 hour at 20oC. The cells were then incubated with ab24102 at a 1/50 dilution for 16 hours at 4oC. The secondary antibody was a Donkey anti-Mouse Alexa Fluor 488, used at a 1/400 dilution.
Overlay histogram showing HL60 cells stained with ab24102 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24102, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Tong WY et al. Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma. J Nanobiotechnology 16:38 (2018). Read more (PubMed: 29653579) »
- Untereiner AA et al. Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells. Biochem Pharmacol 149:174-185 (2018). WB . Read more (PubMed: 29061341) »