Anti-MRP1 antibody [MRPm5] (ab24102)
Key features and details
- Mouse monoclonal [MRPm5] to MRP1
- Suitable for: WB, ICC/IF, IHC-Fr, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MRP1 antibody [MRPm5]
See all MRP1 primary antibodies -
Description
Mouse monoclonal [MRPm5] to MRP1 -
Host species
Mouse -
Specificity
MRPm5 reacts with an internal epitope of MRP1, a 180-195 kD transmembrane transporter protein overexpressed in various
human non-P-glycoprotein MDR tumor cell lines.MRPm5 does not cross-react with the human MDR1 and MDR3 gene products.
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Tested applications
Suitable for: WB, ICC/IF, IHC-Fr, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human MRP1 aa 950-1250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P33527 -
Epitope
MRP1 antibody reacts with an internal epitope of MRP1. -
Positive control
- ICC/IF: Human ES cell derived hepatocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS
Serum free tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
MRPm5 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
Perform heat mediated antigen retrieval in citrate buffer (pH 6.0) for 10 minutes before commencing with IHC staining protocol. |
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| Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval in citrate buffer (pH 6.0) for 10 minutes before commencing with IHC staining protocol. |
|
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency. -
Tissue specificity
Lung, testis and peripheral blood mononuclear cells. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4363 Human
- Omim: 158343 Human
- SwissProt: P33527 Human
- Unigene: 391464 Human
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Alternative names
- ABC 29 antibody
- ABC29 antibody
- ABCC 1 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab24102 has been referenced in 45 publications.
- Gao Q et al. Exosomal lncRNA UCA1 from cancer-associated fibroblasts enhances chemoresistance in vulvar squamous cell carcinoma cells. J Obstet Gynaecol Res 47:73-87 (2021). PubMed: 32812305
- Lahmar A et al. Essential Oils, Pituranthos chloranthus and Teucrium ramosissimum, Chemosensitize Resistant Human Uterine Sarcoma MES-SA/Dx5 Cells to Doxorubicin by Inducing Apoptosis and Targeting P-Glycoprotein. Nutrients 13:N/A (2021). PubMed: 34069490
- Guo H et al. Emodin alleviates gemcitabine resistance in pancreatic cancer by inhibiting MDR1/P-glycoprotein and MRPs expression. Oncol Lett 20:167 (2020). PubMed: 32934734
- Zhang X et al. ID4 Promotes Breast Cancer Chemotherapy Resistance via CBF1-MRP1 Pathway. J Cancer 11:3846-3857 (2020). PubMed: 32328189
- Sun H et al. LncRNA KCNQ1OT1 contributes to the progression and chemoresistance in acute myeloid leukemia by modulating Tspan3 through suppressing miR-193a-3p. Life Sci 241:117161 (2020). PubMed: 31837329