Anti-MRP1 antibody [MRPr1] (ab3368)

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Overview

  • Product name
    Anti-MRP1 antibody [MRPr1]
    See all MRP1 primary antibodies
  • Description
    Rat monoclonal [MRPr1] to MRP1
  • Host species
    Rat
  • Specificity
    This antibody detects MRP 1. It does not cross-react with the human MDR 1 and MDR 3 P glycoprotein gene products.
  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Bacterial fusion protein of human MRP 1 containing a segment of 168 amino acids inthe amino-proximal half of the protein.

  • Epitope
    Internal epitope.
  • Positive control
    • Cell Lines: Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Tissue section: Breast carcinoma

Applications

Our Abpromise guarantee covers the use of ab3368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-Fr 1/20 - 1/50.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
  • Tissue specificity
    Lung, testis and peripheral blood mononuclear cells.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • ABC 29 antibody
      • ABC29 antibody
      • ABCC 1 antibody
      • ABCC antibody
      • Abcc1 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 1 antibody
      • ATP binding cassette sub-family C member 1 antibody
      • ATP binding cassette subfamily C member 1 antibody
      • ATP binding cassette transporter variant ABCC1delta ex13 antibody
      • ATP binding cassette transporter variant ABCC1delta ex13&14 antibody
      • ATP binding cassette transporter variant ABCC1delta ex25 antibody
      • ATP binding cassette transporter variant ABCC1delta ex25&26 antibody
      • ATP binding cassette, sub-family C (CFTR/MRP), member 1 antibody
      • ATP-binding cassette sub-family C member 1 antibody
      • DKFZp686N04233 antibody
      • DKFZp781G125 antibody
      • GS X antibody
      • GSX antibody
      • Leukotriene C(4) transporter antibody
      • LTC4 transporter antibody
      • MRP 1 antibody
      • MRP antibody
      • MRP1 antibody
      • MRP1_HUMAN antibody
      • Multidrug resistance associated protein 1 antibody
      • Multidrug resistance protein antibody
      • Multidrug resistance-associated protein 1 antibody
      • Multiple drug resistance associated protein antibody
      • Multiple drug resistance protein 1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [MRPr1] (ab3368)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP1 antibody [MRPr1] (ab3368)

      ICC/IF image of ab3368 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3368,5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP1 antibody [MRPr1] (ab3368)
      IHC image of ab3368 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:
    • Fan H  et al. Knockdown of long non-coding RNA PVT1 reverses multidrug resistance in colorectal cancer cells. Mol Med Rep 17:8309-8315 (2018). Read more (PubMed: 29693171) »
    • Pan X  et al. Downregulation of eIF4G by microRNA-503 enhances drug sensitivity of MCF-7/ADR cells through suppressing the expression of ABC transport proteins. Oncol Lett 13:4785-4793 (2017). WB . Read more (PubMed: 28599480) »
    See all 7 Publications for this product

    Publishing research using ab3368? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-MRP1 antibody [MRPr1]

     Below Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (MCF7)
    Loading amount
    30 µg
    Specification
    MCF7
    Treatment
    drug or DMSO
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (lysates not heated and no b-mercapto or DTT added to loading buffer)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MRP1 antibody [MRPr1]

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Breast)
    Specification
    Human Breast
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 05 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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