Western blot abreview for Anti-MRP1 antibody [MRPr1]

Below Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (MCF7)
Loading amount
30 µg
Specification
MCF7
Treatment
drug or DMSO
Gel Running Conditions
Reduced Non-Denaturing (Native) (lysates not heated and no b-mercapto or DTT added to loading buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Other product details

Dilution
1/2000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk

Secondary antibody

Name
Non-Abcam antibody was used: anti-rat
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
west pico
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 170 kDa
Positive control
Mock and Anthracycline treated

Additional data

Additional Notes
membranes are frequently dirty with this antibody, relatively long exposure needed. proteins must not be heated or we do not obtain bands, which is a pain for applying mutliple antibodies to the same membrane.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

Abcam user community

Verified customer

Submitted Apr 05 2013

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