Overview

  • Product name

    Anti-MRP2 antibody [EPR10997(2)]
    See all MRP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10997(2)] to MRP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MRP2 aa 1-100. The exact sequence is proprietary.
    Database link: Q92887

  • Positive control

    • Human Liver tissue; Human fetal liver lysate; HepG2 and A549 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10997(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 174 kDa.Can be blocked with Recombinant Human MRP2 protein (ab112272).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
  • Tissue specificity

    Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
  • Involvement in disease

    Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC30 antibody
    • abcC2 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
    • ATP binding cassette subfamily C member 2 antibody
    • ATP-binding cassette sub-family C member 2 antibody
    • Canalicular multidrug resistance protein antibody
    • Canalicular multispecific organic anion transporter 1 antibody
    • CMOAT antibody
    • CMOAT1 antibody
    • cMRP antibody
    • DJS antibody
    • KIAA1010 antibody
    • MRP 2 antibody
    • MRP2_HUMAN antibody
    • Multidrug resistance associated protein 2 antibody
    • Multidrug resistance-associated protein 2 antibody
    see all

Images

  • All lanes : Anti-MRP2 antibody [EPR10997(2)] (ab187644) at 1/1000 dilution

    Lane 1 : Wild-type A549 whole cell lysate
    Lane 2 : ABCC2 knockout A549 whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 174 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab187644 observed at 210 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab187644 was shown to recognize MRP2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab187644 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry analysis of paraffin-embedded Human liver tissue labeling MRP2 with ab187644 at 1/1000 dilution. A prediluted HRP polymer for Rabbit IgG used as secondary antibody. Counterstained with Hematoxylin.

  • All lanes : Anti-MRP2 antibody [EPR10997(2)] (ab187644) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : A549 lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 174 kDa

References

This product has been referenced in:

  • Aiba Y  et al. Increased expression and altered localization of cathepsin Z are associated with progression to jaundice stage in primary biliary cholangitis. Sci Rep 8:11808 (2018). Read more (PubMed: 30087368) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 26 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1mM EDTA 10mM Tris-HCl (pH=9.0)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 26 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 9.0
Sample
Mouse Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 23 2015

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