Overview

  • Product name
    Anti-MRP2 antibody [EPR10998]
    See all MRP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10998] to MRP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MRP2 aa 1500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92887

  • Positive control
    • Human fetal liver, HepG2 and A549 lysates. Permeabilized A549 cells. A549 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10998
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 174 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
    • Tissue specificity
      Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
    • Involvement in disease
      Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
    • Sequence similarities
      Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABC30 antibody
      • abcC2 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
      • ATP binding cassette subfamily C member 2 antibody
      • ATP-binding cassette sub-family C member 2 antibody
      • Canalicular multidrug resistance protein antibody
      • Canalicular multispecific organic anion transporter 1 antibody
      • CMOAT antibody
      • CMOAT1 antibody
      • cMRP antibody
      • DJS antibody
      • KIAA1010 antibody
      • MRP 2 antibody
      • MRP2_HUMAN antibody
      • Multidrug resistance associated protein 2 antibody
      • Multidrug resistance-associated protein 2 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized A549 cells using ab172630 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

    • Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling MRP2 with ab172630 at a 1/250 dilution.

    • All lanes : Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : A549 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 174 kDa

    References

    This product has been referenced in:
    • Warrier A  et al. Efflux transporters and tight junction expression changes in human gastrointestinal cell lines cultured in defined medium vs serum supplemented medium. Life Sci N/A:N/A (2018). WB . Read more (PubMed: 29857072) »
    • Tong XP  et al. Rosemary Extracts Upregulate Nrf2, Sestrin2, and MRP2 Protein Level in Human Hepatoma HepG2 Cells. Evid Based Complement Alternat Med 2017:7359806 (2017). Read more (PubMed: 28286534) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172630.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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