Overview

  • Product name

    Anti-MRP2 antibody [EPR10998]
    See all MRP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10998] to MRP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MRP2 aa 1500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q92887

  • Positive control

    • WB: Human fetal liver, HepG2 and A549 lysates. ICC/IF: HepG2 cells and A549 cells. FC: Permeabilized A549 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10998
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 174 kDa.
ICC/IF 1/100.

For unpurified use at 1/250 - 1/500.

Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/100 - 1/500.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
    • Tissue specificity

      Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
    • Involvement in disease

      Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ABC30 antibody
      • abcC2 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
      • ATP binding cassette subfamily C member 2 antibody
      • ATP-binding cassette sub-family C member 2 antibody
      • Canalicular multidrug resistance protein antibody
      • Canalicular multispecific organic anion transporter 1 antibody
      • CMOAT antibody
      • CMOAT1 antibody
      • cMRP antibody
      • DJS antibody
      • KIAA1010 antibody
      • MRP 2 antibody
      • MRP2_HUMAN antibody
      • Multidrug resistance associated protein 2 antibody
      • Multidrug resistance-associated protein 2 antibody
      see all

    Images

    • All lanes : Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution

      Lane 1 : Wild-type A549 whole cell lysate
      Lane 2 : ABCC2 knockout A549 whole cell lysate
      Lane 3 : HepG2 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 174 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab172630 observed at 210 kDa. Red - loading control, ab130007, observed at 130 kDa.

      ab172630 was shown to recognize MRP2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab172630 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Anti-MRP2 antibody [EPR10998] (ab172630) at 1/2000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 174 kDa
      Observed band size: >190 kDa
      why is the actual band size different from the predicted?



      The multiple bands are consistent with what has been described in PMID: 18832477

    • Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1:100 dilution (7.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling MRP2 with purified ab172630 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • All lanes : Anti-MRP2 antibody [EPR10998] (ab172630) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : A549 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 174 kDa

    • Flow cytometric analysis of permeabilized A549 cells using ab172630 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

    • Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling MRP2 with ab172630 at a 1/250 dilution.

    References

    This product has been referenced in:

    • Warrier A  et al. Efflux transporters and tight junction expression changes in human gastrointestinal cell lines cultured in defined medium vs serum supplemented medium. Life Sci N/A:N/A (2018). WB . Read more (PubMed: 29857072) »
    • Tong XP  et al. Rosemary Extracts Upregulate Nrf2, Sestrin2, and MRP2 Protein Level in Human Hepatoma HepG2 Cells. Evid Based Complement Alternat Med 2017:7359806 (2017). Read more (PubMed: 28286534) »
    See all 2 Publications for this product

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