Overview

  • Product name
    Anti-MRP2 antibody [M2II-12]
    See all MRP2 primary antibodies
  • Description
    Mouse monoclonal [M2II-12] to MRP2
  • Host species
    Mouse
  • Specificity
    ab24106 reacts with MRP2 (ABCC2) and does not cross react with the human MDR1, MRP1, MRP3 and MRP5 gene products.
  • Tested applications
    Suitable for: ICC, Flow Cyt, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein:

    K TFLRHTGPEE EATVHDGSEE EDDDYGLISS VEEIPEDAAS ITMRRENSFR RTLSRSSRSN GRHLKSLRNS LKTRNVNSLK EDEELVKGQK

    , corresponding to Internal sequence amino acids 860-950 of Human MRP2

Properties

Applications

Our Abpromise guarantee covers the use of ab24106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Flow Cyt
WB
IHC-Fr
  • Application notes
    Flow Cyt: Use at an assay dependent dilution.
    ICC: Use at 1/20-1/50 dilution on acetone fixed cytospin preparations.
    IHC-Fr: Use at 1/20 dilution. Acetone fixed.
    WB: 1/20-1/50. Detects a band of approximately 190-200 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
    • Tissue specificity
      Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
    • Involvement in disease
      Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
    • Sequence similarities
      Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABC30 antibody
      • abcC2 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
      • ATP binding cassette subfamily C member 2 antibody
      • ATP-binding cassette sub-family C member 2 antibody
      • Canalicular multidrug resistance protein antibody
      • Canalicular multispecific organic anion transporter 1 antibody
      • CMOAT antibody
      • CMOAT1 antibody
      • cMRP antibody
      • DJS antibody
      • KIAA1010 antibody
      • MRP 2 antibody
      • MRP2_HUMAN antibody
      • Multidrug resistance associated protein 2 antibody
      • Multidrug resistance-associated protein 2 antibody
      see all

    References

    ab24106 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question
    Answer

    Thank you very much for your inquiry and your patience while waiting for our answer.

    I have inquired with the laboratories of the polyclonal antibodies, whether the immunogen sequence is conserved with the pig sequence. This would then provide a good basis for testing the antibody.

    The ab110740 has unfortunately only 79%homology with the pigsequence and we can not recommend it therefore.

    Also for the ab58709, the alignment is only 62%, and we can not recommend it neither. I am very sorry.

    For the monoclonal antibodies, it is more difficult to predict, as the epitopes have not been mapped. As however the overall sequence alignment is relatively good, they might have also good changes to crossreact with the pig protein. Please see here the overall alignment: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20120627-135954-0702-94637034-oy

    The ab3373 and the ab15603 have been raised against the human protein sequence 1339-1541. There are many conserved residues.
    The ab24106 and the ab130385 have been raised against the human sequence 860-950, which seems less conserved than the C terminal. The ab3372 has been raised against human sequence 215 to 310, again, a less conserved region than the C terminus.

    So in summary, I would think either the ab3373 or ab15603 most likely to crossreact with the pig protein from the choice of antibodies we have. This has however not been tested, and it can well be that they will not react with the pig protein.
    https://www.abcam.com/index.html?datasheet=3373 (or use the following: https://www.abcam.com/index.html?datasheet=3373).
    https://www.abcam.com/index.html?datasheet=15603 (or use the following: https://www.abcam.com/index.html?datasheet=15603).

    If you would therefore like to test this, I can offer a discount off a future purchase if you buy ab3373 or ab15603 now, test it inpig and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab3373 or ab15603 in pig. I will then send a discount code. This code must be issued before purchasingthe antibodyso please wait for my reply before ordering.

    2. Purchase ab3373 or ab15603 either by phone, fax, or online (www.abcam.com).

    3. Test it in pig.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab3373 or ab15603 turns out to be unsuitable for pig, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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