Product nameAnti-MRP2 antibody [M2III-5]
See all MRP2 primary antibodies
DescriptionMouse monoclonal [M2III-5] to MRP2
SpecificityThis antibody does not cross-react with the human MDR1 P-gp, MRP1, MRP3, or MRP5 gene products.
Tested applicationsSuitable for: WB, Flow Cyt, ICC, IHC-P, IHC-Frmore details
Species reactivityReacts with: Rat, Human
Does not react with: Mouse
This antibody was raised against a fusion protein of the bacterial maltose binding protein and rat MRP2, containing the 202-amino acid C terminal end of the transporter protein.
Epitopeab15603 reacts with an internal epitope of MRP2; a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: Tissue culture supernatant, 0.7% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab15603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20 - 1/50. Predicted molecular weight: 185.4 kDa.|
|Flow Cyt||Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ICC||1/20 - 1/50. Acetone fixed cytospin preparations.|
|IHC-P||1/20. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/20. Acetone fixed.|
FunctionMediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
Tissue specificityFound on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
Involvement in diseaseDefects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Information by UniProt
- ABC30 antibody
- abcC2 antibody
- ATP binding cassette sub family C (CFTR/MRP) member 2 antibody
All lanes : Anti-MRP2 antibody [M2III-5] (ab15603) at 1/20 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : ABCC2 knockout A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 185.4 kDa
Lanes 1 - 2: Merged signal (red and green). Green - ab15603 observed at 210 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab15603 was shown to recognize ABCC2 in wild-type A549 cells as signal was lost at the expected MW in ABCC2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ABCC2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab15603 and ab52866 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/20 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This product has been referenced in:
- Yang AJ et al. Acute exercise and brain BACE1 protein content: a time course study. Physiol Rep 7:e14084 (2019). Read more (PubMed: 31033251) »
- Baranowski BJ et al. Evaluation of neuropathological effects of a high-fat high-sucrose diet in middle-aged male C57BL6/J mice. Physiol Rep 6:e13729 (2018). Read more (PubMed: 29890051) »