Product nameAnti-MRP3 antibody [M3II-9]
See all MRP3 primary antibodies
DescriptionMouse monoclonal [M3II-9] to MRP3
SpecificityThis antibody does not cross react with MDR1, MRP1, MRP2 or MRP5 gene products
Tested applicationsSuitable for: Flow Cyt, ICC, IHC-Fr, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
EpitopeThis antibody detects with an internal epitope of the membrane transport protein of MRP3
- Cell lines: Drug sensitive parental cell lines and their multidrug resistant derivatives
The rat species was added as a result of positive Abreviews and publication PMID 22112382. In response to few recent complaints, we have decided to remove this species and no longer guarantee it. Please contact firstname.lastname@example.org for any inquiry.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: 0.7% BSA
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab3375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||1/20 - 1/50.
Acetone or formaldehyde fixation is recommended for cytospin preparations.
|IHC-Fr||1/20 - 1/40.
Acetone fixation of sections is recommended.
|WB||1/20 - 1/50. Detects a band of approximately 190 kDa.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21878559|
FunctionMay act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.
Tissue specificityMainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Information by UniProt
- ABC 31 antibody
- ABC31 antibody
- ABCC 3 antibody
HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744) in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.
This product has been referenced in:
- Zukauskas A et al. Transporters MRP1 and MRP2 Regulate Opposing Inflammatory Signals To Control Transepithelial Neutrophil Migration during Streptococcus pneumoniae Lung Infection. mSphere 3:N/A (2018). Read more (PubMed: 29976647) »
- Madden LR et al. Bioprinted 3D Primary Human Intestinal Tissues Model Aspects of Native Physiology and ADME/Tox Functions. iScience 2:156-167 (2018). Read more (PubMed: 30428372) »