• Product name
    Anti-MRP3 antibody [M3II-9]
    See all MRP3 primary antibodies
  • Description
    Mouse monoclonal [M3II-9] to MRP3
  • Host species
  • Specificity
    This antibody does not cross react with MDR1, MRP1, MRP2 or MRP5 gene products
  • Tested applications
    Suitable for: Flow Cyt, ICC, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein:


    , corresponding to amino acids 830-949 of Human MRP3.

  • Epitope
    This antibody detects with an internal epitope of the membrane transport protein of MRP3
  • Positive control
    • Cell lines: Drug sensitive parental cell lines and their multidrug resistant derivatives
  • General notes

    The rat species was added as a result of positive Abreviews and publication PMID 22112382. In response to few recent complaints, we have decided to remove this species and no longer guarantee it. Please contact technical@abcam.com for any inquiry.



Our Abpromise guarantee covers the use of ab3375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


ICC 1/20 - 1/50.

Acetone or formaldehyde fixation is recommended for cytospin preparations.

IHC-Fr 1/20 - 1/40.

Acetone fixation of sections is recommended.

WB 1/20 - 1/50. Detects a band of approximately 190 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 21878559


  • Function
    May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.
  • Tissue specificity
    Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 31 antibody
    • ABC31 antibody
    • ABCC 3 antibody
    • ABCC3 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 3 antibody
    • ATP binding cassette sub family C member 3 antibody
    • ATP-binding cassette sub-family C member 3 antibody
    • Canalicular multispecific organic anion transporter 2 antibody
    • Canicular multispecific organic anion transporter antibody
    • CMOAT 2 antibody
    • CMOAT2 antibody
    • EST90757 antibody
    • MLP 2 antibody
    • MLP2 antibody
    • MOAT D antibody
    • MOAT-D antibody
    • MOATD antibody
    • MRP 3 antibody
    • MRP3_HUMAN antibody
    • Multi specific organic anion transporter D antibody
    • Multi-specific organic anion transporter D antibody
    • Multidrug resistance associated protein 3 antibody
    • Multidrug resistance associated protein antibody
    • Multidrug resistance-associated protein 3 antibody
    • Multispecific organic anion tranporter D antibody
    see all


  •  HepG2 cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of clotrimazole (ab120744) in DMSO. Increased expression of MRP3 (ab3375) correlates with an increase in clotrimazole concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab3375 at 1/50 dilution and ab9484 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.


This product has been referenced in:
  • Zukauskas A  et al. Transporters MRP1 and MRP2 Regulate Opposing Inflammatory Signals To Control Transepithelial Neutrophil Migration during Streptococcus pneumoniae Lung Infection. mSphere 3:N/A (2018). Read more (PubMed: 29976647) »
  • Canet MJ  et al. Altered regulation of hepatic efflux transporters disrupts acetaminophen disposition in pediatric nonalcoholic steatohepatitis. Drug Metab Dispos 43:829-35 (2015). Read more (PubMed: 25788542) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A


Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102.
https://www.abcam.com/index.html?datasheet=29889 https://www.abcam.com/index.html?datasheet=29889.
MRP4 is barely detectable in liver and therefore I recommend to use ab30304 (Prostate (Human) Tissue Lysate - adult normal tissue) as positive control.
https://www.abcam.com/index.html?datasheet=30304 https://www.abcam.com/index.html?datasheet=30304.
With ab63907 we used ab29745 (Placenta (Human) Tissue Lysate - adult normal tissue) successfully as control.
https://www.abcam.com/index.html?datasheet=29745 https://www.abcam.com/index.html?datasheet=29745.
I hope this information is helpful and wish you good luck with your research.

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Thank you for your enquiry and for your patience. Unfortunately, none of these antibodies are available in a conjugated form but we do have a wide range of secondary antibodies to choose from (including ones conjugated to FITC and PE). At the bottom of the online datasheets for each of these primary antibodies, there is a list of compatible secondary antibodies that can be used. We unfortunately can't offer any discounts. All the information that we have regarding these antibodies is located on the online datasheets, and ab10333 and ab3373 have been tested for application in FACS but ab3380 and ab3375 have not to our knowledge. If you have any more questions, please contact us again.

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