Overview

  • Product name
    Anti-MRP4 antibody [2D2A9]
    See all MRP4 primary antibodies
  • Description
    Mouse monoclonal [2D2A9] to MRP4
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human MRP4 aa 631-692. purified recombinant fragment expressed in E coli.
    Sequence:

    LKKDNEESEQ PPVPGTPTLR NRTFSESSVW SQQSSRPSLK DGALESQDTE NVPVTLSEEN RS


    Database link: O15439

  • Positive control
    • Human MRP4 recombinant protein; MRP4 (aa 631-692)-hIgGFc transfected HEK293 cell lysate; A549 cells; Human endometrial cancer tissue; Human bladder cancer tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS

    0.5% protein stabilizer
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    2D2A9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 149 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    May be an organic anion pump relevant to cellular detoxification.
  • Tissue specificity
    Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABCC 4 antibody
    • ABCC4 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
    • ATP binding cassette sub family C member 4 antibody
    • ATP-binding cassette sub-family C member 4 antibody
    • bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody
    • bA464I2.1 antibody
    • Canalicular multispecific organic anion transporter antibody
    • Canalicular multispecific organic anion transporter ABC superfamily antibody
    • EST170205 antibody
    • MOAT B antibody
    • MOAT-B antibody
    • MOATB antibody
    • MRP 4 antibody
    • MRP/cMOAT related ABC transporter antibody
    • MRP/cMOAT-related ABC transporter antibody
    • MRP4_HUMAN antibody
    • Multi specific organic anion transporter B antibody
    • Multi-specific organic anion transporter B antibody
    • Multidrug resistance associated protein 4 antibody
    • Multidrug resistance-associated protein 4 antibody
    • OTTHUMP00000018560 antibody
    see all

Images

  • All lanes : Anti-MRP4 antibody [2D2A9] (ab175364) at 1/500 dilution

    Lane 1 : non transfected HEK293 cell lysate.
    Lane 2 : MRP4 (aa 631-692)-hIgGFc transfected HEK293 cell lysate.

    Predicted band size: 149 kDa



    Expected MW is 32.4 kDa

  • Anti-MRP4 antibody [2D2A9] (ab175364) at 1/500 dilution + Human MRP4 recombinant protein.

    Predicted band size: 149 kDa



    Expected MW is 32.4 kDa

  • Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling MRP4 using ab175364 at 1/200 followed by DAB staining.

  • Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling MRP4 using ab175364 at 1/200 followed by DAB staining.

  • Flow cytometric analysis of A549 cells labeling MRP4 using ab175364 at 1/200 (green) and negative control (red).

References

ab175364 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175364.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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