Overview

  • Product name

  • Description

    Goat polyclonal to MRP4
  • Host species

    Goat
  • Specificity

    ab77184 is expected to recognize MRP4 isoform 1 (NP_005836.2) only.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding with sequence CNGQPSTLTIFETAL from the C terminal region of the human MRP4 protein (NP_005836.2)

  • Positive control

    • Lysates prepared from rat renal cortex membranes. Human prostate tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab77184 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 150 kDa).
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Function

    May be an organic anion pump relevant to cellular detoxification.
  • Tissue specificity

    Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABCC 4 antibody
    • ABCC4 antibody
    • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
    • ATP binding cassette sub family C member 4 antibody
    • ATP-binding cassette sub-family C member 4 antibody
    • bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody
    • bA464I2.1 antibody
    • Canalicular multispecific organic anion transporter antibody
    • Canalicular multispecific organic anion transporter ABC superfamily antibody
    • EST170205 antibody
    • MOAT B antibody
    • MOAT-B antibody
    • MOATB antibody
    • MRP 4 antibody
    • MRP/cMOAT related ABC transporter antibody
    • MRP/cMOAT-related ABC transporter antibody
    • MRP4_HUMAN antibody
    • Multi specific organic anion transporter B antibody
    • Multi-specific organic anion transporter B antibody
    • Multidrug resistance associated protein 4 antibody
    • Multidrug resistance-associated protein 4 antibody
    • OTTHUMP00000018560 antibody
    see all

Images

  • Anti-MRP4 antibody (ab77184) at 0.5 µg/ml + rat renal cortex membranes at 20 µg

    Predicted band size: 150 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?

  • ab77184 (4µg/ml) staining of paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • ab77184, at 2.5 µg/ml, staining MRP4 in paraffin embedded human prostate tissue by Immunohistochemistry. Steamed antigen retrievalUse of ab77184 was followed by AP staining.

References

This product has been referenced in:

  • Castella B  et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9Vd2 T cell activation by dendritic cells. Nat Commun 8:15663 (2017). Read more (PubMed: 28580927) »
  • Chapy H  et al. Blood-brain and retinal barriers show dissimilar ABC transporter impacts and concealed effect of P-glycoprotein on a novel verapamil influx carrier. Br J Pharmacol 173:497-510 (2016). Read more (PubMed: 26507673) »
See all 6 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab24102.
https://www.abcam.com/index.html?datasheet=29889 https://www.abcam.com/index.html?datasheet=29889.
MRP4 is barely detectable in liver and therefore I recommend to use ab30304 (Prostate (Human) Tissue Lysate - adult normal tissue) as positive control.
https://www.abcam.com/index.html?datasheet=30304 https://www.abcam.com/index.html?datasheet=30304.
With ab63907 we used ab29745 (Placenta (Human) Tissue Lysate - adult normal tissue) successfully as control.
https://www.abcam.com/index.html?datasheet=29745 https://www.abcam.com/index.html?datasheet=29745.
I hope this information is helpful and wish you good luck with your research.

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