RecombinantRabMAb

Recombinant Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
  • Immunoprecipitation - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
  • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
  • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
  • Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20403] to MRP4 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-MRP4 antibody [EPR20403] - BSA and Azide free
    See all MRP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR20403] to MRP4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human MRP4 aa 1050 to the C-terminus. The exact sequence is proprietary.
    Database link: O15439

  • Positive control

    • IHC-P: Human ovarian cancer tissue.

  • General notes

    Ab235624 is the carrier-free version of ab233382. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab235624 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab235624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 220-250 kDa (predicted molecular weight: 150 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    May be an organic anion pump relevant to cellular detoxification.

  • Tissue specificity

    Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ABCC 4 antibody
      • ABCC4 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
      • ATP binding cassette sub family C member 4 antibody
      • ATP-binding cassette sub-family C member 4 antibody
      • bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody
      • bA464I2.1 antibody
      • Canalicular multispecific organic anion transporter antibody
      • Canalicular multispecific organic anion transporter ABC superfamily antibody
      • EST170205 antibody
      • MOAT B antibody
      • MOAT-B antibody
      • MOATB antibody
      • MRP 4 antibody
      • MRP/cMOAT related ABC transporter antibody
      • MRP/cMOAT-related ABC transporter antibody
      • MRP4_HUMAN antibody
      • Multi specific organic anion transporter B antibody
      • Multi-specific organic anion transporter B antibody
      • Multidrug resistance associated protein 4 antibody
      • Multidrug resistance-associated protein 4 antibody
      • OTTHUMP00000018560 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Basolateral membrane staining on human prostate epithelia (PMID:18615486, PMID:25840885). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. MRP4 is expressed at the apical (brush border) membrane of human kidney tubules (PMID: 11856762). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunoprecipitation - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      MRP4 was immunoprecipitated from 0.35 mg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate with ab233382 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233382 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: U-2 OS whole cell lysate 10 µg (Input).

      Lane 2: ab233382 IP in U-2 OS whole cell lysate (+).

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233382 in U-2 OS whole cell lysate (-).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      The molecular mass observed is consistent with the literature (PMID: 15827327).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

    • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling MRP4 with ab233382 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the A549 cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

    • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling MRP4 with ab233382 at 1/50 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the LNCaP cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human ovarian cancer cells is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab235624? Please let us know so that we can cite the reference in this datasheet.

    ab235624 has not yet been referenced specifically in any publications.

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