Recombinant
RabMAb

Recombinant Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

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Overview

  • Product name

    Anti-MRP4 antibody [EPR20403] - BSA and Azide free
    See all MRP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR20403] to MRP4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human MRP4 aa 1050 to the C-terminus. The exact sequence is proprietary.
    Database link: O15439

  • Positive control

    • IHC-P: Human ovarian cancer tissue.

  • General notes

    Ab235624 is the carrier-free version of ab233382. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab235624 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 220-250 kDa (predicted molecular weight: 150 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    May be an organic anion pump relevant to cellular detoxification.

  • Tissue specificity

    Widely expressed, with particularly high levels in prostate, but is barely detectable in liver.

  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ABCC 4 antibody
      • ABCC4 antibody
      • ATP binding cassette sub family C (CFTR/MRP) member 4 antibody
      • ATP binding cassette sub family C member 4 antibody
      • ATP-binding cassette sub-family C member 4 antibody
      • bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody
      • bA464I2.1 antibody
      • Canalicular multispecific organic anion transporter antibody
      • Canalicular multispecific organic anion transporter ABC superfamily antibody
      • EST170205 antibody
      • MOAT B antibody
      • MOAT-B antibody
      • MOATB antibody
      • MRP 4 antibody
      • MRP/cMOAT related ABC transporter antibody
      • MRP/cMOAT-related ABC transporter antibody
      • MRP4_HUMAN antibody
      • Multi specific organic anion transporter B antibody
      • Multi-specific organic anion transporter B antibody
      • Multidrug resistance associated protein 4 antibody
      • Multidrug resistance-associated protein 4 antibody
      • OTTHUMP00000018560 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Basolateral membrane staining on human prostate epithelia (PMID:18615486, PMID:25840885). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. MRP4 is expressed at the apical (brush border) membrane of human kidney tubules (PMID: 11856762). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunoprecipitation - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      MRP4 was immunoprecipitated from 0.35 mg U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate with ab233382 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233382 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: U-2 OS whole cell lysate 10 µg (Input).

      Lane 2: ab233382 IP in U-2 OS whole cell lysate (+).

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233382 in U-2 OS whole cell lysate (-).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      The molecular mass observed is consistent with the literature (PMID: 15827327).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

    • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling MRP4 with ab233382 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the A549 cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

    • Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunocytochemistry/ Immunofluorescence - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling MRP4 with ab233382 at 1/50 dilution, followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the LNCaP cell line.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [EPR20403] - BSA and Azide free (ab235624)

      Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling MRP4 with ab233382 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human ovarian cancer cells is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233382).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab235624 has not yet been referenced specifically in any publications.

    Publishing research using ab235624? Please let us know so that we can cite the reference in this datasheet.

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