Product nameAnti-MRP5 antibody [M5II-54]
See all MRP5 primary antibodies
DescriptionRat monoclonal [M5II-54] to MRP5
Specificityab24107 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products
Tested applicationsSuitable for: IHC-Fr, Flow Cyt, ICC/IF, IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Fusion protein, corresponding to amino acids 722-910 of Human MRP5.
Epitopeab24107 reacts with an internal epitope of MRP5. ab24107 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products
ab24107 has potential value for detection of MRP5-mediated drug-resistance in human tumor samples
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: 0.7% BSA
Serum free tissue culture supernatant
PurityTissue culture supernatant
Purification notesAntibody containing supernatant has been concentrated and filtered through a 0.22 micron filter and is serum free.
Primary antibody notesab24107 has potential value for detection of MRP5-mediated drug-resistance in human tumor samples
Our Abpromise guarantee covers the use of ab24107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/20 - 1/50.|
|IP||Use at an assay dependent concentration. PubMed: 19323627|
|IHC-P||Use at an assay dependent concentration. PubMed: 19323627|
|WB||1/20 - 1/50. Predicted molecular weight: 161 kDa.|
FunctionActs as a multispecific organic anion pump which can transport nucleotide analogs.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Information by UniProt
- ABC 33 antibody
- ABC33 antibody
- ABCC 5 antibody
Overlay histogram showing Y79 cells stained with ab24107 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24107, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H&L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Y79 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Zhang H et al. Loss of miR-516a-3p mediates upregulation of ABCC5 in prostate cancer and drives its progression. Onco Targets Ther 11:3853-3867 (2018). Read more (PubMed: 30013366) »
- Kataoka J et al. Loss of Runt-related transcription factor 3 induces resistance to 5-fluorouracil and cisplatin in hepatocellular carcinoma. Oncol Rep 35:2576-82 (2016). IHC . Read more (PubMed: 26985715) »