Anti-MRP5 antibody [M5II-54] (ab24107)
Key features and details
- Rat monoclonal [M5II-54] to MRP5
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MRP5 antibody [M5II-54]
See all MRP5 primary antibodies -
Description
Rat monoclonal [M5II-54] to MRP5 -
Host species
Rat -
Specificity
ab24107 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human MRP5 aa 700-950. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Epitope
ab24107 reacts with an internal epitope of MRP5. ab24107 does not cross-react with the human MDR1, MRP1, MRP2 or MRP3 gene products -
General notes
ab24107 has potential value for detection of MRP5-mediated drug-resistance in human tumor samples
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituent: 0.7% BSA
Serum free tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Antibody containing supernatant has been concentrated and filtered through a 0.22 micron filter and is serum free. -
Primary antibody notes
ab24107 has potential value for detection of MRP5-mediated drug-resistance in human tumor samples -
Clonality
Monoclonal -
Clone number
M5II-54 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 1µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Acts as a multispecific organic anion pump which can transport nucleotide analogs. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10057 Human
- Entrez Gene: 27416 Mouse
- Entrez Gene: 116721 Rat
- Omim: 605251 Human
- SwissProt: O15440 Human
- SwissProt: Q9R1X5 Mouse
- SwissProt: Q9QYM0 Rat
- Unigene: 368563 Human
see all -
Alternative names
- ABC 33 antibody
- ABC33 antibody
- ABCC 5 antibody
see all
Images
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Overlay histogram showing Y79 cells stained with ab24107 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24107, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H&L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Y79 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab24107 has been referenced in 7 publications.
- Gu YH et al. Combined BRM270 and endostatin inhibit relapse of NSCLC while suppressing lung cancer stem cell proliferation induced by endostatin. Mol Ther Oncolytics 22:565-573 (2021). PubMed: 34553041
- Guo H et al. Emodin alleviates gemcitabine resistance in pancreatic cancer by inhibiting MDR1/P-glycoprotein and MRPs expression. Oncol Lett 20:167 (2020). PubMed: 32934734
- Lu Y et al. HNF1A inhibition induces the resistance of pancreatic cancer cells to gemcitabine by targeting ABCB1. EBioMedicine 44:403-418 (2019). PubMed: 31103629
- Zhang H et al. Loss of miR-516a-3p mediates upregulation of ABCC5 in prostate cancer and drives its progression. Onco Targets Ther 11:3853-3867 (2018). PubMed: 30013366
- Kataoka J et al. Loss of Runt-related transcription factor 3 induces resistance to 5-fluorouracil and cisplatin in hepatocellular carcinoma. Oncol Rep 35:2576-82 (2016). IHC . PubMed: 26985715
- Messenger SW et al. Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway. J Biol Chem 289:28040-53 (2014). PubMed: 25138214
- Karla PK et al. Expression of multidrug resistance associated protein 5 (MRP5) on cornea and its role in drug efflux. J Ocul Pharmacol Ther 25:121-32 (2009). IP, IHC-P ; Human . PubMed: 19323627