Overview

  • Product name
    Anti-MRP8 + MRP14 antibody [27E10]
    See all MRP8 + MRP14 primary antibodies
  • Description
    Mouse monoclonal [27E10] to MRP8 + MRP14
  • Host species
    Mouse
  • Specificity
    Does not react with lymphocytes or platelets.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Fr, IHC-Pmore details
    Unsuitable for: FuncS
  • Species reactivity
    Reacts with: Human, Rhesus monkey
    Does not react with: Mouse
  • Immunogen

    Human blood monocytes.

  • Epitope
    ab17050 recognises an epitope on the MRP8 + MRP14 complex which is not exposed on the individual subunits.
  • Positive control
    • Human granulocytes
  • General notes

    ab17050 can be used for early detection of inflammatory macrophages, characterisation of tumorous tissues and monitoring of peripheral blood cell cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab17050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/10. Perform under non-denaturing conditions only.
IP Use at an assay dependent concentration.
Flow Cyt 1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr 1/10.
IHC-P Use at an assay dependent concentration. PubMed: 21517798
  • Application notes
    Is unsuitable for FuncS.
  • Target

    • Relevance
      MRP8 and MRP14 are members of the S100 family of proteins containing 2 EF hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21.
    • Cellular localization
      Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • CAGA antibody
      • CAGB antibody
      • Calgranulin A antibody
      • Calgranulin B antibody
      • Calprotectin L1L subunit antibody
      • CFAG antibody
      • CP10 antibody
      • Cystic fibrosis antigen antibody
      • Leukocyte L1 complex light chain antibody
      • Macrophage L1 protein Calprotectin antibody
      • Migration inhibitory factor related protein 14 antibody
      • Migration inhibitory factor related protein 8 antibody
      • MRP-14 antibody
      • MRP-8 antibody
      • P14 antibody
      • P8 antibody
      • S100 calcium binding protein A8 antibody
      • S100 calcium binding protein A9 antibody
      • S100A8 antibody
      • S100A9 antibody
      • Urinary stone protein band A antibody
      see all

    Images

    • Flow cytometric detection of Calprotectin with ab17050. The green and black line represent the isotype control in 2 concentrations, the pink line the secondary antibody only and the green and brown line ab17050 in a concentration of 1 and 3 μl/200000 cells.

    References

    This product has been referenced in:
    • Dai J  et al. Intracellular S100A9 Promotes Myeloid-Derived Suppressor Cells during Late Sepsis. Front Immunol 8:1565 (2017). Read more (PubMed: 29204146) »
    • Val S  et al. Proteomic Characterization of Middle Ear Fluid Confirms Neutrophil Extracellular Traps as a Predominant Innate Immune Response in Chronic Otitis Media. PLoS One 11:e0152865 (2016). IF . Read more (PubMed: 27078692) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you for your enquiry.

    I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any MRP8 and MRP14 antibody tested in the species and application you are using should be suitable for your applications.

    Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).

    For your experiment, maybe an antibody that will bind to both proteins will be suitable? ab17050 recognises an epitope on the MRP8 + MRP14 complex which is not exposed on the individual subunits.

    https://www.abcam.com/index.html?datasheet=17050 (or use the following: https://www.abcam.com/index.html?datasheet=17050).

    If you prefer two separate antibodies, I can recommend rabbit monoclonals over mouse monoclonals:

    http://www.epitomics.com/advantage/

    ab92331
    https://www.abcam.com/index.html?datasheet=92331 (or use the following: https://www.abcam.com/index.html?datasheet=92331).

    ab92507
    https://www.abcam.com/index.html?datasheet=92507 (or use the following: https://www.abcam.com/index.html?datasheet=92507).


    I hope this information is helpful. Should you have any further questions please do not hesitate to ask.

    Read More

    Question
    Answer

    Thank you for contacting us.

    The supplying lab send me the following information:

    Unfortunately we do not have a detailled protocol for use on paraffin sections. The application has been added in the past upon personal communication by the scientists who developed the antibody. Please note that Calprotectin is only expressed in inflammatory tissue and even then it depends at which time point in the inflammatory process tissue sections are obtained. We would recommend to carefully read thearticle mentioned on the datasheet to set up initial experiments with right positive control using frozen sections. If that protocol is optimal we would then try to transfer to paraffin sections of tissues obtained under similar conditions using standard IHC protocol/antigen retrieval.

    The article is:

    Zwadlo G et al. A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues. J Immunol 137:512-8 (1986). PubMed: 3722815





    As a general guideline, I would suggest starting with citrate buffer as antigen retrieval.



    I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Oral Dysplastic Epithelium cells)
    Specification
    Oral Dysplastic Epithelium cells
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Enzymatic
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 2%

    Mr. Cancer center

    Verified customer

    Submitted Jul 25 2006

    Answer

    For the generation of the antibody human blood monocytes are used. We regret to inform you that we have no more information about the immunogen. Furthermore we have no information about the reactivity with rat Calprotectin. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). Please let me know if you require any further assistance.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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