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RecombinantRabMAb

Recombinant Anti-MRP8 antibody [EPR3553] (ab126721)

  • Datasheet
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Product price, shipping and contact information

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Western blot - Anti-MRP8 antibody [EPR3553] (ab126721)
  • Anti-MRP8 antibody [EPR3553] (ab126721)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3553] to MRP8
  • Suitable for: WB
  • Reacts with: Human

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Protein
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Recombinant Human MRP8 protein (ab167749)
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-MRP8 antibody [EPR3553]
    See all MRP8 primary antibodies
  • Description

    Rabbit monoclonal [EPR3553] to MRP8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human MRP8 (P05109).

  • Positive control

    • HL-60 lysates Human spleen lysates PBMC lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3553
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Cancer
    • Drug resistance
    • MRP-related proteins

Associated products

  • Alternative Versions

    • Anti-MRP8 antibody [EPR3553] - BSA and Azide free (ab248147)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HL60 whole cell lysate (ab7914)
  • Recombinant Protein

    • Recombinant Human MRP8 protein (ab167749)

Applications

Our Abpromise guarantee covers the use of ab126721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
    • Tissue specificity

      Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
    • Sequence similarities

      Belongs to the S-100 family.
      Contains 2 EF-hand domains.
    • Cellular localization

      Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
    • Target information above from: UniProt accession P05109 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6279 Human
      • Omim: 123885 Human
      • SwissProt: P05109 Human
      • Unigene: 416073 Human
      • Alternative names

        • 60B8Ag antibody
        • AI323541 antibody
        • B8Ag antibody
        • BEE11 antibody
        • CAGA antibody
        • Calgranulin-A antibody
        • Calprotectin L1L subunit antibody
        • Calprotectin, included antibody
        • CFAG antibody
        • CGLA antibody
        • Chemotactic cytokine CP-10 antibody
        • CP-10 antibody
        • Cystic fibrosis antigen antibody
        • L1Ag antibody
        • Leukocyte L1 complex light chain antibody
        • MA387 antibody
        • MIF antibody
        • Migration inhibitory factor-related protein 8 antibody
        • MRP-8 antibody
        • Myeloid-related protein 8 antibody
        • Neutrophil cytosolic 7 kDa protein antibody
        • NIF antibody
        • p8 antibody
        • Pro-inflammatory S100 cytokine antibody
        • Protein S100-A8 antibody
        • S100 calcium binding protein A8 (calgranulin A) antibody
        • S100 calcium binding protein A8 antibody
        • S100 calcium-binding protein A8 antibody
        • S100A8 antibody
        • S100A8/S100A9 complex, included antibody
        • S10A8_HUMAN antibody
        • Urinary stone protein band A antibody
        see all

      Images

      • Western blot - Anti-MRP8 antibody [EPR3553] (ab126721)
        Western blot - Anti-MRP8 antibody [EPR3553] (ab126721)
        All lanes : Anti-MRP8 antibody [EPR3553] (ab126721) at 1/1000 dilution

        Lane 1 : HL-60 lysates
        Lane 2 : Human spleen lysates
        Lane 3 : PBMC lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 10 kDa

      • Anti-MRP8 antibody [EPR3553] (ab126721)
        Anti-MRP8 antibody [EPR3553] (ab126721)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab126721? Please let us know so that we can cite the reference in this datasheet.

      ab126721 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Looking for dog specific anti S100A8 and S100A9 antibodies.

      Read More

      Abcam community

      Verified customer

      Asked on Jul 12 2013

      Answer

      I have checked the immunogen compatibility of all MRP8 antibodies - 2 antibodies ab75519 and ab126721 has homology more than 70% with human protein so these antibodies will be worth trying. I am happy to offer you Abtrial codes for these. Please check the below link for more information;

      https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

      ab62227 is the anti S100A9 antibodies and it was tested with Dog species. The other antibodies which may detect dog protein is ab92507 which has sequence homology more than 70%.

      Please choose any of these antibodies and contact me know for the Abtrial code.

      Read More

      Padamjeet Singh

      Abcam Scientific Support

      Answered on Jul 12 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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