- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MRP8 antibody [EPR3553]
See all MRP8 primary antibodies -
DescriptionRabbit monoclonal [EPR3553] to MRP8
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivityReacts with: Human
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Immunogen
A synthetic peptide corresponding to residues in Human MRP8 (P05109).
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Positive control
- HL-60 lysates Human spleen lysates PBMC lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3553
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 10 kDa. | |
| IP | 1/10 - 1/100. |
Target
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FunctionCalcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
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Tissue specificityExpressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
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Sequence similaritiesBelongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localizationSecreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
- Information by UniProt
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Database links
- Entrez Gene: 6279 Human
- Omim: 123885 Human
- SwissProt: P05109 Human
- Unigene: 416073 Human
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Alternative names
- 60B8Ag antibody
- AI323541 antibody
- B8Ag antibody
see all
Images
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![Western blot - Anti-MRP8 antibody [EPR3553] (ab126721)](https://www.abcam.com/ps/datasheet/images/126/ab126721/MRP8-Primary-antibodies-ab126721-1.jpg)
Western blot - Anti-MRP8 antibody [EPR3553] (ab126721)All lanes : Anti-MRP8 antibody [EPR3553] (ab126721) at 1/1000 dilution
Lane 1 : HL-60 lysates
Lane 2 : Human spleen lysates
Lane 3 : PBMC lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 10 kDa
References
ab126721 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"