Name: Anti-MRP8 antibody [EPR3554]
Brand: RabMAb
SKU: ab92331
Rating: 5 (6 reviews)

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Western blot - Anti-MRP8 antibody [EPR3554] (ab92331)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3554] to MRP8
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Human

Biotin Carrier Free HRP

Product name

Anti-MRP8 antibody [EPR3554]
See all MRP8 primary antibodies
Description

Rabbit monoclonal [EPR3554] to MRP8
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide within Human MRP8 aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P05109
Positive control
- WB: Human (adult and fetal) and mouse spleen tissue lysates and PMBC and HL-60 cell lysates. IHC-P: Human cervical carcinoma, breast carcinoma, tonsil and spleen tissues. IP: HL-60 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3554
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human MRP8 protein (ab167749)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(4)	1/1000. Predicted molecular weight: 11 kDa. For unpurified use at 1/2000 - 1/10000.
IP		1/30. For unpurified use at 1/20.
IHC-P	(1)	1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250.

Notes
WB 1/1000. Predicted molecular weight: 11 kDa. For unpurified use at 1/2000 - 1/10000.
IP 1/30. For unpurified use at 1/20.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt or ICC/IF.

Function

Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
Tissue specificity

Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
Sequence similarities

Belongs to the S-100 family.
Contains 2 EF-hand domains.
Cellular localization

Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
Target information above from: UniProt accession P05109 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6279 Human
- Entrez Gene: 20201 Mouse
- Omim: 123885 Human
- SwissProt: P05109 Human
- SwissProt: P27005 Mouse
- Unigene: 416073 Human
- Unigene: 21567 Mouse
Alternative names
- 60B8Ag antibody
- AI323541 antibody
- B8Ag antibody
- BEE11 antibody
- CAGA antibody
- Calgranulin-A antibody
- Calprotectin L1L subunit antibody
- Calprotectin, included antibody
- CFAG antibody
- CGLA antibody
- Chemotactic cytokine CP-10 antibody
- CP-10 antibody
- Cystic fibrosis antigen antibody
- L1Ag antibody
- Leukocyte L1 complex light chain antibody
- MA387 antibody
- MIF antibody
- Migration inhibitory factor-related protein 8 antibody
- MRP-8 antibody
- Myeloid-related protein 8 antibody
- Neutrophil cytosolic 7 kDa protein antibody
- NIF antibody
- p8 antibody
- Pro-inflammatory S100 cytokine antibody
- Protein S100-A8 antibody
- S100 calcium binding protein A8 (calgranulin A) antibody
- S100 calcium binding protein A8 antibody
- S100 calcium-binding protein A8 antibody
- S100A8 antibody
- S10A8_HUMAN antibody
- Urinary stone protein band A antibody
see all

Western blot - Anti-MRP8 antibody [EPR3554] (ab92331)

All lanes : Anti-MRP8 antibody [EPR3554] (ab92331) at 1/20000 dilution (purified)

Lane 1 : HL-60 (Human promyelocytic leukemia cell line) cell lysate
Lane 2 : Human fetal spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labeling MRP8 with purified ab92331 at 1/500.

Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunoprecipitation - Anti-MRP8 antibody [EPR3554] (ab92331)

ab92331 (purified) at 1/30 immunoprecipitating MRP8 in HL-60 (Human promyelocytic leukemia cell line) cell lysate (Lane 1).

Lane 2 - rabbit monoclonal IgG instead of ab92331 in HL-60 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling MRP8 with unpurified ab92331.

Detection: DAB staining.

Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-MRP8 antibody [EPR3554] (ab92331)

Anti-MRP8 antibody [EPR3554] (ab92331) at 1/2000 dilution (purified) + Mouse spleen tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling MRP8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-MRP8 antibody [EPR3554] (ab92331)

All lanes : Anti-MRP8 antibody [EPR3554] (ab92331) (unpurified)

Lane 1 : Human spleen tissue lysate
Lane 2 : PBMC cell lysate
Lane 3 : HL-60 (Human promyelocytic leukemia cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling MRP8 with unpurified ab92331.

Detection: DAB staining.

Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MRP8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Anti-MRP8 antibody [EPR3554] (ab92331)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab92331? Please let us know so that we can cite the reference in this datasheet.

ab92331 has been referenced in 34 publications.

Leimkühler NB et al. Heterogeneous bone-marrow stromal progenitors drive myelofibrosis via a druggable alarmin axis. Cell Stem Cell 28:637-652.e8 (2021). PubMed: 33301706
Wang Y et al. Differential proteomic analysis of tibial subchondral bone from male and female guinea pigs with spontaneous osteoarthritis. Exp Ther Med 21:633 (2021). PubMed: 33968164
Zhu G et al. Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts. Syst Biol Reprod Med 66:59-69 (2020). PubMed: 31714804
Zouboulis CC et al. Alterations in innate immunity and epithelial cell differentiation are the molecular pillars of hidradenitis suppurativa. J Eur Acad Dermatol Venereol 34:846-861 (2020). PubMed: 31838778
Li Z et al. Proinflammatory S100A8 Induces PD-L1 Expression in Macrophages, Mediating Tumor Immune Escape. J Immunol 204:2589-2599 (2020). PubMed: 32198140

View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application

Western blot

Sample

Human Cell lysate - whole cell (Skin epidermoid A431)

Gel Running Conditions

Reduced Denaturing (15%)

Loading amount

40 µg

Specification

Skin epidermoid A431

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 20 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Skin epidermal layer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer, pH 6.0

Specification

Skin epidermal layer

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Mar 20 2018

Application

Western blot

Sample

Human Tissue lysate - whole (epithelium, liver)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris)

Loading amount

15 µg

Specification

epithelium, liver

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 02 2017

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Human Tissue sections (Gastric biopsy)

Antigen retrieval step

Heat mediated

Permeabilization

Specification

Gastric biopsy

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted May 13 2016

Application

Western blot

Sample

Human Cell lysate - other (Human gingival crevicular fluid)

Gel Running Conditions

Reduced Denaturing (4-15% gel)

Loading amount

5 µg

Specification

Human gingival crevicular fluid

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 24 2015

Application

Western blot

Sample

Human Cell lysate - whole cell (human neutrophil)

Loading amount

20 µg

Specification

human neutrophil

Treatment

10mM FMLP for 10min

Gel Running Conditions

Reduced Denaturing (15%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 07 2012

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (34)

Customer reviews and Q&As

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Western blot abreview for Anti-MRP8 antibody [EPR3554]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MRP8 antibody [EPR3554]

Western blot abreview for Anti-MRP8 antibody [EPR3554]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MRP8 antibody [EPR3554]

Western blot abreview for Anti-MRP8 antibody [EPR3554]

Western blot abreview for Anti-MRP8 antibody [EPR3554]